FIGURE

Figure 9

ID
ZDB-FIG-191230-33
Publication
Lechermeier et al., 2019 - Transcript Analysis of Zebrafish GLUT3 Genes, slc2a3a and slc2a3b, Define Overlapping as Well as Distinct Expression Domains in the Zebrafish (Danio rerio) Central Nervous System
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Figure 9

RNA in situ hybridization of gad1b and double RNA in situ hybridization for slc2a3a and gad1b, a marker for GABAergic neurons. For comparison, a single in situ hybridization for gad1b was performed at 24 hpf (A,B,B’) and 36 hpf (C,D,D’) old zebrafish embryos. Boxes in (A,B,B’,C,D,D’) depict location of high magnification pictures a1,a2,b1,b2 and c1, c2, d1, d2, respectively. In addition, a 24 hpf old zebrafish embryo was stained with slc2a3a DIG in situ probe (blue) and gad1b FLUO probe (red). Upper rows show lateral views (A,C,E) and lower rows ventral views (B,B’,D,D’,F). Anterior is to the left. Boxes in (E,F) depict location of high magnification pictures e1–e4 and f1–f4, respectively. Boxes in e1 and e2 and f1, f2, and f4) represent higher magnification pictures shown in e1’, e2’ and f1’, f2’, and f4’. Box in e1’ represents location of even higher magnification shown in e1”. White arrows mark double-positive cells or cell populations. Red arrow marks an example of cells only positive for gad1b and blue arrows cells only positive for slc2a3a. Scale bars in (A,E), 100 μm and pertains to (A–F); scale bar in a1, 50 μm and valid for a1–d2; scale bar in e1, 50 μm and pertains to e1–e2 and f1–2; scale bar in e3, 50 μm and valid for e3-e4, and f3–4, scale bars in e1’–f4’, e1’, 10 μm.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.