Lin et al., 2015 - Anion Exchanger 1b in Stereocilia Is Required for the Functioning of Mechanotransducer Channels in Lateral-Line Hair Cells of Zebrafish. PLoS One   10:e0117041 Full text @ PLoS One

Fig. 1

In situ hybridization and immunocytochemistry of anion exchanger 1b (AE1b) in zebrafish larvae.

AE1b mRNA was expressed by ionocytes (short arrows) and neuromasts (long arrows) of 72-h post-fertilization (hpf) larvae (A, B). No signal was found in larval skin with the sense probe (C). Confocal images of lateral-line neuromasts with the zebrafish AE1b antibody (D, E). AE1b was expressed on the apical portion of neuromasts in 96-hpf larvae (arrows, D). Magnification of the neuromast apical membrane showing that AE1b was expressed in stereocilia (arrows, E). The morphology of stereocilia was verified by an actin antibody (arrows, F).

Anatomical Terms:
Stage Range: Protruding-mouth to Day 4

Fig. 2

Effects of morpholino knockdown of zAE1b on neuromast hair cell.

Double staining of zAE1b (red; A-C) and parvalbumin (Par; green; B, D) antibodies was conducted in control (Con; A, B) and morpholino knockdown (MO; C, D) 3 dpf larvae. B and D: merged images of zAE1b and Par protein signals. (E) The Ca2+ influx of neuromast hair cells in zAE1b morpholino knockdown larvae was significantly decreased. Data are presented as the mean ± SE. *Significant difference (Student’s t test, p<0.05). Numbers in parentheses are numbers of neuromasts.

Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth

Fig. 3

Effect of DIDS on Ca2+ influx of neuromasts.

(A) Sequential recording of Ca2+ influx of neuromast hair cells in 96-hpf larvae before and after the addition of 300 μM DIDS. (B) Larvae were pre-incubated in normal water with 200 or 300 μM DIDS for 30 min. Then the influx was recorded in normal recording medium without DIDS. Data are presented as the mean ± SE. a, b, c Indicate a significant difference (by one-way ANOVA, Tukey’s comparison, p < 0.05). Numbers in parentheses are numbers of neuromasts.

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