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ZIRC
ZFIN ID: ZDB-FIG-150415-13
Lin et al., 2015 - Anion Exchanger 1b in Stereocilia Is Required for the Functioning of Mechanotransducer Channels in Lateral-Line Hair Cells of Zebrafish. PLoS One   10:e0117041 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 2

Effects of morpholino knockdown of zAE1b on neuromast hair cell.

Double staining of zAE1b (red; A-C) and parvalbumin (Par; green; B, D) antibodies was conducted in control (Con; A, B) and morpholino knockdown (MO; C, D) 3 dpf larvae. B and D: merged images of zAE1b and Par protein signals. (E) The Ca2+ influx of neuromast hair cells in zAE1b morpholino knockdown larvae was significantly decreased. Data are presented as the mean ± SE. *Significant difference (Student’s t test, p<0.05). Numbers in parentheses are numbers of neuromasts.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb IHC AB control Protruding-mouth neuromast hair cell
IHC AB + MO1-slc4a1b standard conditions Protruding-mouth neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-slc4a1b standard conditions Protruding-mouth neuromast hair cell morphology, normal
Protruding-mouth neuromast hair cell calcium ion import decreased efficacy, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One