|ZFIN ID: ZDB-FIG-150415-13|
Effects of morpholino knockdown of zAE1b on neuromast hair cell.
Double staining of zAE1b (red; A-C) and parvalbumin (Par; green; B, D) antibodies was conducted in control (Con; A, B) and morpholino knockdown (MO; C, D) 3 dpf larvae. B and D: merged images of zAE1b and Par protein signals. (E) The Ca2+ influx of neuromast hair cells in zAE1b morpholino knockdown larvae was significantly decreased. Data are presented as the mean ± SE. *Significant difference (Student’s t test, p<0.05). Numbers in parentheses are numbers of neuromasts.
|Ab1-pvalb||IHC||AB||control||Protruding-mouth||neuromast hair cell|
|IHC||AB + MO1-slc4a1b||standard conditions||Protruding-mouth||neuromast hair cell|
|AB + MO1-slc4a1b||standard conditions||Protruding-mouth||neuromast hair cell calcium ion import decreased efficacy, abnormal|
|Protruding-mouth||neuromast hair cell morphology, normal|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One|