Ishak et al., 2008 - Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish. Reproductive Biology and Endocrinolgy   6:56 Full text @ Reprod. Biol. Endocrinol.

Fig. 2 Validation of semi-quantitative RT-PCR assays for fadsd6 (desaturase), elovl5 (elongase) and h2afz (histone H2A) genes.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult

Fig. 3 Expression of elovl5 and fadsd6 normalized to h2afz during the zebrafish follicles development from PV to ovulation stage. PV represents pre-vitellogenic stage, EV represents early and mid-vitellogenic stage, LV represents fully-grown but immature stage, M represents matured oocyte stage and O represents ovulated oocyte stage. - ve represents negative control, with sterile water as template for RT-PCR. Each value represents mean ± SEM of three PCR runs at the significant level P < 0.05 using Tukey's HSD. Mean values with different alphabets are significantly different. Representative of the electrophoretic images of RT-PCR is shown at the lower panel.

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.
Open Access.
Full text @ Reprod. Biol. Endocrinol.