Ishak et al., 2008 - Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish. Reproductive Biology and Endocrinolgy   6:56 Full text @ Reprod. Biol. Endocrinol.

Fig. 2 Validation of semi-quantitative RT-PCR assays for fadsd6 (desaturase), elovl5 (elongase) and h2afz (histone H2A) genes.

Anatomical Term:
Stage: Adult

Fig. 3 Expression of elovl5 and fadsd6 normalized to h2afz during the zebrafish follicles development from PV to ovulation stage. PV represents pre-vitellogenic stage, EV represents early and mid-vitellogenic stage, LV represents fully-grown but immature stage, M represents matured oocyte stage and O represents ovulated oocyte stage. - ve represents negative control, with sterile water as template for RT-PCR. Each value represents mean ± SEM of three PCR runs at the significant level P < 0.05 using Tukey's HSD. Mean values with different alphabets are significantly different. Representative of the electrophoretic images of RT-PCR is shown at the lower panel.

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Open Access.
Full text @ Reprod. Biol. Endocrinol.