ZFIN ID: ZDB-FIG-090127-68
Ishak et al., 2008 - Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish. Reproductive Biology and Endocrinolgy   6:56 Full text @ Reprod. Biol. Endocrinol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 3 Expression of elovl5 and fadsd6 normalized to h2afz during the zebrafish follicles development from PV to ovulation stage. PV represents pre-vitellogenic stage, EV represents early and mid-vitellogenic stage, LV represents fully-grown but immature stage, M represents matured oocyte stage and O represents ovulated oocyte stage. - ve represents negative control, with sterile water as template for RT-PCR. Each value represents mean ± SEM of three PCR runs at the significant level P < 0.05 using Tukey's HSD. Mean values with different alphabets are significantly different. Representative of the electrophoretic images of RT-PCR is shown at the lower panel.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
elovl5 WT standard conditions Adult oocyte RTPCR
Adult ovarian follicle stage I RTPCR
Adult ovarian follicle stage II RTPCR
Adult ovarian follicle stage III RTPCR
Adult ovarian follicle stage IV RTPCR
fads2 WT standard conditions Adult ovarian follicle stage I RTPCR
Adult ovarian follicle stage II RTPCR
Adult ovarian follicle stage III RTPCR
Adult ovarian follicle stage IV RTPCR
h2az2a WT standard conditions Adult oocyte RTPCR
Adult ovarian follicle stage I RTPCR
Adult ovarian follicle stage II RTPCR
Adult ovarian follicle stage III RTPCR
Adult ovarian follicle stage IV RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Open Access.
Full text @ Reprod. Biol. Endocrinol.