Transgenic Construct

Tg(myo6b:gria2a-tmie-2A-NLS-mCherry)

ID
ZDB-TGCONSTRCT-190823-3
Name
Tg(myo6b:gria2a-tmie-2A-NLS-mCherry)
Previous Names
  • Tg(myo6b:gria2a.SP-tmie.44.231-p2A-RLS(mCherry)) (1)
  • Tg(myo6b:SP44-231-p2A-NLS(mCherry)) (1)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
[Em λ][Ex λ]
Fpbase:mCherry
Contains Sequences
None
Note
In this construct, the myo6b promoter drives expression in sensory hair cells of a construct encoding an N-terminal truncated form of Tmie, in which the predicted first transmembrane helix of Tmie has been replaced with a known signal peptide (SP) from the zebrafish glutamate receptor Gria2a. The unrelated signal peptide serves to preserve the predicted membrane topology of Tmie. mCherry expression is directed to the nuclei of sensory hair cells
Plasmid Map
None
Genomic Features
That Utilize tg(myo6b:gria2a-tmie-2a-nls-mcherry) Construct
Genomic Feature Affected Genomic Regions
zf2152Tg
Transgenics
That Utilize tg(myo6b:gria2a-tmie-2a-nls-mcherry) Construct
Sequence Information
Other Construct Pages
None
Citations