ZFIN ID: ZDB-TGCONSTRCT-190823-3
Transgenic Construct Name: Tg(myo6b:gria2a-tmie-2A-NLS-mCherry)
Sequence Ontology ID :
Synonyms: Tg(myo6b:gria2a.SP-tmie.44.231-p2A-RLS(mCherry)) (1), Tg(myo6b:SP44-231-p2A-NLS(mCherry)) (1)

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Note: In this construct, the myo6b promoter drives expression in sensory hair cells of a construct encoding an N-terminal truncated form of Tmie, in which the predicted first transmembrane helix of Tmie has been replaced with a known signal peptide (SP) from the zebrafish glutamate receptor Gria2a. The unrelated signal peptide serves to preserve the predicted membrane topology of Tmie. mCherry expression is directed to the nuclei of sensory hair cells

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CONSTRUCT COMPONENTS
Regulatory Regions: myo6b (1)
Coding Sequences: mCherry (1)
2A (1), NLS (1)
gria2a (1), tmie (1)
GENOMIC FEATURES THAT UTILIZE Tg(myo6b:gria2a-tmie-2A-NLS-mCherry)
Genomic Feature Affected Genomic Regions    
zf2152Tg    
TRANSGENICS THAT UTILIZE Tg(myo6b:gria2a-tmie-2A-NLS-mCherry)
Fish Affected Genomic Regions Phenotype Gene Expression
tmieru1000/ru1000 ; vo28Tg ; zf2152Tg(TU) tmie Fig. 8 with image from Pacentine et al., 2019
tmieru1000/ru1000 ; zf2152Tg(TU) tmie
SEQUENCE INFORMATION No data available
OTHER Tg(myo6b:gria2a-tmie-2A-NLS-mCherry) TRANSGENIC CONSTRUCT PAGES
CITATIONS (1)