IMAGE

Fig. 4

ID
ZDB-IMAGE-211118-44
Genes
Antibodies
Source
Figures for Guglielmi et al., 2021
Image
Figure Caption

Fig. 4

The Nodal–pSmad2 signaling gradient forms independently of Smad4a.

a Maximum intensity projection (MIP) of quantitative ISH for lft1 at mid gastrulation (50–60% epiboly) in CTRL and MZsmad4a embryos treated ±10 µM SB-505124 at sphere stage. Scale bars correspond to 80 µm. Lateral views are shown. Nuclei were stained with DAPI (white). b Quantitation of a. Orange, dark blue, and magenta dots show segmented cells from CTRL and MZsmad4a embryos treated ±10 µM SB-505124. n = 8 embryos for each group. c Curve fitting for segmented cell intensities shown in b. Means are shown by black lines and the orange/dark blue shading indicates the SEMs. d, e MIP of quantitative ISH for ndr1 in CTRL and MZsmad4a embryos at mid-gastrulation (50–60% epiboly). Regions indicated in d by white boxes are shown enlarged in e. Scale bars correspond to 80 µm in d and 20 µm in e. Arrowheads indicate YSL. Lateral views are shown. Nuclei were stained with DAPI (white). f Quantitation of d. Orange and dark blue dots show segmented cells from CTRL and MZsmad4a embryos. n = 8 embryos for each group. g Curve fitting for segmented cell intensities shown in f. Means are shown by black lines and the orange/dark blue shading indicates the SEMs. h MIP of CTRL and MZsmad4a at 40% epiboly, immunostained for pSmad2. Scale bar corresponds to 80 µm. Lateral views are shown. Nuclei were stained with DAPI (blue). i Quantitation of the pSmad2 immunostaining at the margin of CTRL and MZsmad4a embryos. Each trace represents n = 8 embryos. Means are shown by black lines and the orange/dark blue shading indicates the SEMs. Dotted line shows baseline.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.