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Fig. 6 Expression of apical epidermal fold markers and en-1 in wildtype and syu mutant pectoral fin buds. Wholemount in situ hybridizations. Anterior is to the left and distal to the top, except in (Q-T), where dorsal is to the left and distal the top. (A,B,K,L,Q,R) 32 hour pectoral fin buds; (C,D,G,H,M,N) 38 hour pectoral fin buds; (E,F,I,J,O,P,S,T) 48 hour pectoral fin buds. (A,C,E,G,I,K,M,O,Q,S) Wild-type fin buds; (B,D,F,H,J,L,N,P,R,T) syu fin buds. (A-F) dlx-2 RNA; (G-J) fgf-8 RNA; (K-P) bmp-2 RNA; (Q-T) en-1 RNA. dlx-2 and bmp- 2 expression appears normal in the apical epidermis of syu buds at 32 hours, but is reduced at 38 hours and 48 hours. Note that dlx-2 and bmp-2 expression is stronger in the posterior apical epidermis of wild-type, but not syu buds at 38 hours. bmp-2 is expressed in posterior mesenchyme at 32 hours in syu buds, but this expression is lost by 38 hours. fgf-8 expression is not detectable in syu fin buds. en-1 is expressed normally in syu fin buds at 32 and 48 hours. Note the apical thickening in the wild-type bud (arrowhead in S), which is not visible in the syu bud (arrowhead in T).