Fig. 1

Fig. 1 Mutants of ldd239 display granulopoiesis and angiogenesis defects. ((A)–(D)) WISH analysis of mpx expression at 18 hpf and 22 hpf. In ldd239 mutants, mpx has an obviously decreased expression in the ICM compared with wild-type siblings at 18 hpf ((A) and (B), black arrows) and 22 hpf ((C) and (D), black arrows). Black arrows indicate the mpx+ cells in the ICM. White arrows represent the mpx+ cells in the A-LPM which have no significant difference between siblings and mutants ((C) and (D), white arrows). ((E)–(J)) WISH assays of fli1 and flk1 expression at 18 hpf and 22 hpf. Expression of fli1 has no difference between siblings and mutants at 18 hpf ((E) and (F)). Expression of fli1 and flk1 at 22 hpf show deficient angiogenesis and defects are better shown in higher magnifications ((G)–(J), black arrows). ((K)–(N)) Expression of lyz ((K) and (L)) and l-plastin ((M) and (N)) are normal in the A-LPM of mutants at 22 hpf. ((O)–(T)) Hematopoietic progenitor marker, scl ((O) and (P)), erythrocyte progenitors marker, gata1 ((Q) and (R)), and mature erythrocyte marker, hemoglobin hbae1, expression of all markers have no change in mutants at 22 hpf.