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ZFIN ID: ZDB-IMAGE-101011-38
Figures for Rai et al., 2010


Figure Caption/Comments:

Fig. 2

APC Negatively Regulates Demethylase Expression via Retinoic Acid

(A) Whole-mount in situ staining for aid, gadd457alpha;, and mbd4 in apcmcr and apcwt embryos treated with DMSO, all-trans retinoic acid (ATRA, 1 μM), or Cox-2 inhibitor NS-398 (10 μM) or injected with control morpholino or ctbp1 morpholino (2 ng). β-catenin knockdown efficiency with NS-398 treatment is depicted in Figure S2A.

(B and C) Quantitative RT-PCR for aid, mbd4, and gadd45α in apcmcr and apcwt embryos treated with DMSO or ATRA (B) or in different RA deficiency models in zebrafish, as shown in (C). Expression of genes are first normalized to 28S and then to the control embryo mRNA/28S ratio, valued as 1.

(D) MeDIP-qPCR for genes shown (as in Figure 1E) in apc mutants (72 hpf) that are either untreated or treated with ATRA.

Error bars indicate ± SD. See also Figure S2.

Figure Data:
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