|ZFIN ID: ZDB-IMAGE-050120-8|
OPTICS: MAGNIFICATION: DATE OF IMAGE: SUBMITTER COMMENTS:
Fig. 5 Knockdown of Tsg function partially suppresses the chordino ventralization. (A) Uninjected chordino homozygote; arrowheads indicate multiple ventral fin folds, also seen in a slightly different view at higher magnification (inset). Appearance of multiple fin folds is suppressed by injection of 8 ng MO1 (B, inset shows dorsal view of tail of same embryo). (C-H) Injection of 25 ng MO5 into chordino homozygotes results in a substantial enlargement of dorsally derived tissues. Examination of live embryos at the six-somite stage showed reduced head neural tissue in C and suppression of the reduction in F (arrowheads). (C) Uninjected chordino homozygote; (F) injected chordino homozygote; lateral views, anterior is upwards. In situ analysis at the same stage with pax2.1 (arrowhead), krox20 (bracket) (D,G) and myod (E,H) showed that the somites, the MHB and rhombomeres 3 and 5 are increased in size in the injected (n=14/20) compared with uninjected mutants. (D,E) Uninjected mutants; (G,H) two views of the same injected mutant. In the injected mutant, the anterior neural and somitic mesoderm appears similar to wild type; however, the tail bud is still enlarged, indicating that the dino phenotype is not fully suppressed.
|Acknowledgments:||This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.|