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Gene Trap Construct

Tg(GAL4-VP16,UAS:EGFP)

ID
ZDB-GTCONSTRCT-070314-1
Name
Tg(GAL4-VP16,UAS:EGFP)
Previous Names
  • SAGVG (1)
  • Gt(Gal4-VP16,UAS:EGFP)
  • Gt(Gal4-VP16;UAS:EGFP)
  • Tg(Gal4-VP16,UAS:EGFP)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
Contains Sequences
None
Note
A Tol2 transposon-based, bipartite construct consisting of a Gal4-VP16 gene/enhancer trap as well as a cis-linked UAS-regulated eGFP reporter. The vector contains a splice acceptor sequence derived from the rabbit beta-globin gene (275 base pairs of intron 2 and 45 base pairs of exon 3) which was amplified from the pT2KXIG”IN vector (Urasaki et al., 2006) and cloned upstream of tandem Gal4-VP16 and UAS:eGFP elements (Koster and Fraser, 2001). The entire unit was subcloned into the pT2KXIG”IN vector to be flanked by a 535 bp right Tol2 arm and a 517 bp left Tol2 arm.
Plasmid Map
None
Genomic Features
That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct
Transgenics
That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct
Fish Affected Genomic Regions Phenotype Gene Expression
c215Tg(AB)
c218Tg(AB)
Fig. 1 from Goll et al., 2009
c223Tg(AB)
Fig. 1 from Goll et al., 2009
c223Tg/+; c264Tg/+
Fig. 5 from Davison et al., 2007
c228Tg(AB)
c229Tg(AB)
c230Tg(AB)
c230Tg/+; c264Tg/+
Fig. 5 from Davison et al., 2007
c233Tg(AB)
c235Tg(AB)
c236Tg(AB)
c237Tg(AB)
c239Tg(AB)
c240Tg(AB)
Fig. 1 from Goll et al., 2009
c241Tg(AB)
c247Tg(AB)
c251Tg(AB)
Fig. 1 from Goll et al., 2009
c269Tg
Fig. 5 with image from Feng et al., 2010
c269Tg(AB)
2 figures from Goll et al., 2009
cy13Tg ; icam3as31Gt Fig. 1 with image from Hsieh et al., 2018
icam3as31Gt Fig. 2 with image from Hsieh et al., 2018 Fig. 1 with image from Hsieh et al., 2018
ikbkgc220Gt/c220Gt(AB) Fig. S2 from Davison et al., 2007 Fig. 1 from Goll et al., 2009
ikbkgc220Gt(AB)
ub120Tg
ub120Tg/+(AB)
ub120Tg; zf153Tg
Fig. 1 with image from Zhao et al., 2009
ub121Tg/+(AB)
ub148Tg/+(AB)
ub14Tg/+(AB)
ub15Tg/+(AB)
ub17Tg/+(AB)
ub28Tg/+(AB)
ub38Tg/+(AB)
ub39Tg/+(AB)
ub3Tg
5 figures with image from Zhao et al., 2009
ub3Tg/+(AB)
ub3Tg; c264Tg
Fig. 7 with image from Zhao et al., 2009 Fig. 7 with image from Zhao et al., 2009
ub3Tg; zf153Tg
ub43Tg
5 figures with image from Zhao et al., 2009
ub43Tg/+(AB)
ub43Tg; c264Tg
Fig. 7 with image from Zhao et al., 2009 Fig. 7 with image from Zhao et al., 2009
ub43Tg; s1993tTg
ub43Tg; zf153Tg
ub44Tg/+(AB)
ub45Tg/+(AB)
ub46Tg
ub49Tg
2 figures from Gharbi et al., 2012
ub49Tg; zf153Tg
ub61Tg/+(AB)
ub65Tg/+(AB)
ub67Tg/+(AB)
ub68Tg
ub79Tg/+(AB)
ub80Tg/+(AB)
ub95Tg/+(AB)
uhrf1hi272Tg/hi272Tg; c269Tg Fig. 5 with image from Feng et al., 2010
y1Tg ; icam3as31Gt
Sequence Information
Other Construct Pages
None
Citations