Gene Trap Construct

Tg(GAL4-VP16,UAS:EGFP)

ID
ZDB-GTCONSTRCT-070314-1
Name
Tg(GAL4-VP16,UAS:EGFP)
Previous Names
  • SAGVG (1)
  • Gt(Gal4-VP16,UAS:EGFP)
  • Gt(Gal4-VP16;UAS:EGFP)
  • Tg(Gal4-VP16,UAS:EGFP)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
[Em λ][Ex λ]
Fpbase:EGFP
Contains Sequences
None
Note
A Tol2 transposon-based, bipartite construct consisting of a Gal4-VP16 gene/enhancer trap as well as a cis-linked UAS-regulated eGFP reporter. The vector contains a splice acceptor sequence derived from the rabbit beta-globin gene (275 base pairs of intron 2 and 45 base pairs of exon 3) which was amplified from the pT2KXIG”IN vector (Urasaki et al., 2006) and cloned upstream of tandem Gal4-VP16 and UAS:eGFP elements (Koster and Fraser, 2001). The entire unit was subcloned into the pT2KXIG”IN vector to be flanked by a 535 bp right Tol2 arm and a 517 bp left Tol2 arm.
Plasmid Map
None
Genomic Features
That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct
Transgenics
That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct
Fish Affected Genomic Regions Phenotype Gene Expression
c215Tg (AB)
c218Tg (AB)
Fig. 1 from Goll et al., 2009
c223Tg (AB)
Fig. 1 from Goll et al., 2009
c223Tg/+; c264Tg/+
Fig. 5 from Davison et al., 2007
c228Tg (AB)
c229Tg (AB)
c230Tg (AB)
c230Tg/+; c264Tg/+
Fig. 5 from Davison et al., 2007
c233Tg (AB)
c235Tg (AB)
c236Tg (AB)
c237Tg (AB)
c239Tg (AB)
c240Tg (AB)
Fig. 1 from Goll et al., 2009
c241Tg (AB)
c247Tg (AB)
c251Tg (AB)
Fig. 1 from Goll et al., 2009
c264Tg; ub3Tg
Fig. 7 with image from Zhao et al., 2009 Fig. 7 with image from Zhao et al., 2009
c264Tg; ub43Tg
Fig. 7 with image from Zhao et al., 2009 Fig. 7 with image from Zhao et al., 2009
c269Tg
Fig. 5 with image from Feng et al., 2010
c269Tg (AB)
2 figures from Goll et al., 2009
icam3as31Gt Fig. 2 with image from Hsieh et al., 2018 Fig. 1 with image from Hsieh et al., 2018
icam3as31Gt; cy13Tg Fig. 1 with image from Hsieh et al., 2018
icam3as31Gt; y1Tg
ikbkgc220Gt/c220Gt (AB) Fig. S2 from Davison et al., 2007 Fig. 1 from Goll et al., 2009
ikbkgc220Gt (AB)
s1993tTg; ub43Tg
ub120Tg
ub120Tg/+ (AB)
ub120Tg; zf153Tg
Fig. 1 with image from Zhao et al., 2009
ub121Tg/+ (AB)
ub148Tg/+ (AB)
ub14Tg/+ (AB)
ub15Tg/+ (AB)
ub17Tg/+ (AB)
ub28Tg/+ (AB)
ub38Tg/+ (AB)
ub39Tg/+ (AB)
ub3Tg
5 figures with image from Zhao et al., 2009
ub3Tg/+ (AB)
ub3Tg; zf153Tg
ub43Tg
5 figures with image from Zhao et al., 2009
ub43Tg/+ (AB)
ub43Tg; zf153Tg
ub44Tg/+ (AB)
ub45Tg/+ (AB)
ub46Tg
ub49Tg
2 figures from Gharbi et al., 2012
ub49Tg; zf153Tg
ub61Tg/+ (AB)
ub65Tg/+ (AB)
ub67Tg/+ (AB)
ub68Tg
ub79Tg/+ (AB)
ub80Tg/+ (AB)
ub95Tg/+ (AB)
uhrf1hi272Tg/hi272Tg; c269Tg Fig. 5 with image from Feng et al., 2010
Sequence Information
Other Construct Pages
None
Citations