header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180731-1
Hsieh et al., 2018 - Zebrafish VCAP1X2 regulates cardiac contractility and proliferation of cardiomyocytes and epicardial cells. Scientific Reports   8:7856 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 1

The gene trap transgenic zebrafish was characterized as a VCAP1X2 mutant. (A) A diagram of the VCAP1X2 gene trap mutant is shown. Blue boxes indicate exons I-V. White boxes indicate 5′ and 3′ UTRs. Red arrows indicate forward (F) and reverse (R) RT-PCR primers. Yellow triangles indicate 535-bp right Tol2 arm (R) or 517-bp left Tol2 arm (L). UAS, Gal4-responsive upstream activating sequence; E1b, adenoviral E1b minimal promoter element. (B) Splicing of exon 1 and exon 2 was disrupted in VCAP1X2 mutant (mut) embryos at 24 and 48 hpf. (C) Normal morphology of VCAP1X2 mutants and WT embryos at 48 hpf. EGFP expression was detected in the heart (h), posterior cardinal vein (pcv) and primordial hindbrain channel (phbc) of VCAP1X2 mutant (n = 50 for 48 hpf). Scale bar, 25 μm. (D) Expression of EGFP and VCAP1X2 (red) is shown in heart ventricles of Tg(myl7: EGFP; myl7: H2AFZ mcherry) embryos at 96 hpf. No VCAP1X2 expression was detected in the ventricle of homozygous VCAP1X2 mutant heart (n = 15 per condition, N = 3). Scale bar, 50 μm. (E) Expression of VCAP1X2 at shield (a), 5 somite (s) (b), 18 s (c,d), 24 (e–g), 30 (h–j), 48 (k,l) and 72 (m) hpf stages. VCAP1X2 was co-expressed with myl7 in the myocardium of embryos at 24 and 30 hpf (g,j). Scale bars, 30 μm. Co-localization of VCAP1X2 and GFP was detected in phbc (n,o) and pcv (p,q) of Tg(fli1:GFP) embryos at 48 hpf (n = 10). Arrows indicate heart. a, atrium; h, heart; v, ventricle. Scale bars, 50 μm. (F) (a) VCAP1X2 (red) was expressed in the plasma membrane of ventricular myocardium cardiomyocytes labeled with EGFP in Tg(myl7:EGFP; myl7:H2AFZ mCherry) transgenic embryos at 96 hpf. (b) VCAP1X2 was not expressed in EGFP-labeled ventricular epicardium cells in Tg(tcf21:NTR; tcf21:nucEGFP) transgenic embryos at 96 hpf. (c) VCAP1X2 was also not expressed in the EGFP-labeled ventricular endocardium of Tg(fli1:GFP) transgenic embryos at 96 hpf (n = 15 per condition, N = 3). Nuclei were stained by DAPI. Scale bar, 5 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP cy13Tg standard conditions Day 4 cardiac ventricle IFL
cy13Tg ; icam3as31Gt standard conditions Day 4 cardiac ventricle IFL
icam3as31Gt standard conditions Long-pec heart IFL
Long-pec posterior cardinal vein IFL
Long-pec primordial hindbrain channel IFL
pd108Tg; pd41Tg standard conditions Day 4 ventricular endocardium IFL
y1Tg standard conditions Day 4 ventricular endocardium IFL
icam3 WT standard conditions Shield whole organism ISH
5-9 somites whole organism ISH
14-19 somites heart primordium ISH
14-19 somites whole organism ISH
Prim-5 heart tube ISH
Prim-5 posterior cardinal vein ISH
Prim-5 primordial hindbrain channel ISH
Prim-5 whole organism RTPCR
Prim-15 heart tube ISH
Prim-15 posterior cardinal vein ISH
Prim-15 primordial hindbrain channel ISH
Long-pec atrium ISH
Long-pec cardiac ventricle ISH
Long-pec heart ISH
Long-pec posterior cardinal vein ISH
Long-pec primordial hindbrain channel ISH
Long-pec whole organism RTPCR
Protruding-mouth atrium ISH
Protruding-mouth cardiac ventricle ISH
Protruding-mouth heart ISH
Protruding-mouth posterior cardinal vein ISH
Protruding-mouth primordial hindbrain channel ISH
cy13Tg standard conditions Day 4 cardiac ventricle IHC
cy13Tg ; icam3as31Gt standard conditions Day 4 Not Detected cardiac ventricle IHC
icam3as31Gt standard conditions Prim-5 Not Detected whole organism RTPCR
Long-pec Not Detected whole organism RTPCR
pd108Tg; pd41Tg standard conditions Day 4 Not Detected ventricular endocardium IHC
y1Tg standard conditions Long-pec posterior cardinal vein IHC
Long-pec primordial hindbrain channel IHC
Day 4 Not Detected ventricular endocardium IHC
mCherry cy13Tg standard conditions Day 4 cardiac ventricle IFL
myl7 WT standard conditions Prim-5 myocardium ISH
Prim-15 myocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cy13Tg ; icam3as31Gt standard conditions Day 4 cardiac ventricle icam3 expression absent, abnormal
icam3as31Gt standard conditions Prim-5 whole organism icam3 expression absent, abnormal
Long-pec whole organism icam3 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.