ZFIN ID: ZDB-GENE-030813-2
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Gene Name: piwi-like RNA-mediated gene silencing 1
Gene Symbol: piwil1    Nomenclature History

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(Including Attributions)
Gene Type: protein_coding_gene
Location: Chr: 8 Mapping Details/Browsers
Description: Exhibits piRNA binding activity. Involved in several processes, including gene silencing by RNA; germ cell development; and piRNA metabolic process. Localizes to the P granule. Is expressed in several structures, including blastodisc; central nervous system; eye; reproductive system; and unfertilized egg. Orthologous to several human genes including PIWIL1 (piwi like RNA-mediated gene silencing 1).
Genome Resources: Alliance (1),  Gene:368200 (1),  Ensembl(GRCz11):ENSDARG00000041699 (2)
GENE EXPRESSION
All Expression Data: 26 figures from 19 publications
Wild-type Stages, Structures: Zygote:1-cell (0.0h-0.75h) to Adult (90d-730d, breeding adult)
 
Curated Microarray Expression: GEO (1) , Expression Atlas (1)
MUTATIONS AND SEQUENCE TARGETING REAGENTS
Allele Type Localization Consequence Mutagen Suppliers
fh219 Point Mutation Unknown Premature Stop ENU
  • China Zebrafish Resource Center (CZRC) (order this)
  • Zebrafish International Resource Center (ZIRC) (order this)
    • fh220 Point Mutation Unknown Unknown ENU
    fh221 Point Mutation Unknown Unknown ENU
    hu2410 Point Mutation Unknown Unknown ENU
    hu2479 Point Mutation Unknown Premature Stop ENU
    la016400Tg Transgenic Insertion Unknown Unknown DNA
  • Zebrafish International Resource Center (ZIRC) (order this)
    • la026576Tg Transgenic Insertion Unknown Unknown DNA
    piwil1_unrecovered Point Mutation Unknown Unknown ENU
    sa7619 Point Mutation Unknown Missense ENU
    sa8531 Point Mutation Unknown Premature Stop ENU
  • European Zebrafish Resource Center (EZRC) (order this)
  • Zebrafish International Resource Center (ZIRC) (order this)
    • sa30904 Point Mutation Unknown Premature Stop ENU
  • Zebrafish International Resource Center (ZIRC) (order this)
    • sa45333 Point Mutation Unknown Premature Stop ENU
  • Zebrafish International Resource Center (ZIRC) (order this)
    • PHENOTYPE
    Data: 2 figures from Houwing et al., 2007
    Observed in:
    DISEASE ASSOCIATED WITH piwil1 HUMAN ORTHOLOG No data available
    DISEASE ASSOCIATED WITH piwil1 VIA EXPERIMENTAL MODELS No data available
    GENE ONTOLOGY
    Ontology GO Term
    Biological Process anatomical structure morphogenesis (more)
    Cellular Component cytoplasm (more)
    Molecular Function piRNA binding (more)
    GO Terms (all 22)
    PROTEIN FAMILIES, DOMAINS AND SITES
    Type InterPro ID Name
    Domain IPR003100 PAZ domain
    Domain IPR003165 Piwi domain
    Homologous_superfamily IPR012337 Ribonuclease H-like superfamily
    Domain IPR031320 GAGE
    Family IPR031326 Piwi-like protein 1
    Homologous_superfamily IPR036085 PAZ domain superfamily
    Homologous_superfamily IPR036397 Ribonuclease H superfamily
    TRANSCRIPTS
    Type Name Length (nt) Analysis
    mRNA piwil1-203 (1)    Ensembl 549
    Select Tool
    piwil1-204 (1)    Ensembl 762
    Select Tool
    piwil1-205 (1)    Ensembl 3257
    Select Tool
    piwil1-206 (1)    Ensembl 915
    Select Tool
    ncRNA piwil1-006 (1)    Ensembl 898
    Select Tool
    Browsers: UCSC,  NCBI,  Ensembl,  ZFIN
    INTERACTIONS AND PATHWAYS
    ANTIBODIES
    Name Type Isotype Host Organism Assay Source Publications
    Ab2-piwil1 polyclonal Rabbit 1
    Ab1-piwil1 polyclonal Rabbit IHC, WB 6
    PLASMIDS No data available
    CONSTRUCTS WITH SEQUENCES FROM piwil1
    Construct Regulatory Regions Coding Sequences Species Tg Lines Publications
    Tg(piwil1:CFP-Eco.NfsB) piwil1 CFP Danio rerio,  Escherichia coli 1 2
    Tg(piwil1:DsRed) piwil1 DsRed Danio rerio 1 3
    Tg(piwil1:EGFP) piwil1 EGFP Danio rerio 2 8
    Tg(piwil1:EGFP-UTRnanos3) piwil1 EGFP,  nanos3,  UTR Danio rerio 1 3
    Tg(piwil1:NPT) piwil1 NPT Danio rerio 1 3
    Tg(piwil1:tdrd6-mCherry) piwil1 mCherry,  tdrd6 Danio rerio 1 1
    MARKER RELATIONSHIPS
    piwil1 Contained in: [BAC] CH73-308B14 (1), DKEY-245G2 (1) (order this)
    SEQUENCE INFORMATION
    Type Accession # Length (bp/aa) Analysis
    RNA RefSeq:NM_183338 (1) 3273 bp
    Select Tool
    Genomic GenBank:CR450819 (1) 168244 bp
    Select Tool
    Polypeptide UniProtKB:Q8UVX0 (1) 858 aa
    Select Tool
    Sequence Information (all 16)
    ORTHOLOGY for piwil1 ( Chr: 8 )
    CITATIONS (85)