Fig. 7

Fst is required for the LR patterning of Chick and Xenopus. (A) Expression pattern of FST in chick embryos at indicated stages. (B) Expression of NODAL in WT and FST MO transfected chick embryos at the indicated stages. (C) Schematic diagram of experimental setup for (D) expression of NODAL in chick embryos implanted with BSA- or rhFst-soaked beads on the left side of the embryos at the indicated stages. (E) Expression patterns of fst in Xenopus embryos at indicated stages. (A and B, D and E) Numbers in the bottom right of panels indicate the number of embryos with the phenotype shown out of the total number of embryos examined. At least four embryos were examined in each experiment. (F) Schematic diagram of experimental setup for (G) expression of nodal1 in Xenopus tropicalis embryos injected with fst gRNAs + Cas9 or (I) with fst mRNA into one cell at the two-cell stage. Uninjected embryos were used as control. Red triangles indicate the expression of nodal1. (H and J) Statistics of (G) and (I), “n” indicates the numbers of examined embryo. At least 41 embryos were examined in each experiment. (Scale bar, 200 μm.)