Fig. 2

Loss of or gain of function of fsta affects the LR patterning of the Nodal signaling pathway in zebrafish. (A) LR asymmetric genes spaw in the LPM, lefty1 (lft1) and ndr2 in the forebrain analyzed using WISH in WT and fstaΔ5/Δ5 embryos. Grey stacks indicate absence of expression. At least 22 embryos were examined in each experiment. (B) Expression areas of spaw in WT and fstaΔ5/Δ5 embryos were calculated. myod1 was used to indicate the developmental stage. **P < 0.01. At least 10 embryos were examined in each experiment. (A and B) Statistics are shown on the right of the representative photos, “n” indicates the numbers of examined embryos. (C) Schematic diagram of experimental setup for (D) expression of spaw and lefty1 in the light-induced fsta overexpression embryos. (E) Schematic diagram of experimental setup for (F) expression of spaw in zebrafish embryos implanted with Bovine Serum Albumin (BSA)- or rhFST-soaked beads in the left side at the indicated stages. Red triangles indicate the anterior boundary of spaw expression. (D and F) Numbers in the bottom right of panels indicate the number of embryos with the phenotype shown out of the total number of embryos examined. At least a total of 22 embryos were examined in each experiment. (Scale bar, 200 μm.)