PUBLICATION
A spatiotemporal barrier formed by Follistatin is required for left-right patterning
- Authors
- Fu, X.X., Zhuo, D.H., Zhang, Y.J., Li, Y.F., Liu, X., Xing, Y.Y., Huang, Y., Wang, Y.F., Cheng, T., Wang, D., Chen, S.H., Chen, Y.J., Jiang, G.N., Lu, F.I., Feng, Y., Huang, X., Ma, J., Liu, W., Bai, G., Xu, P.F.
- ID
- ZDB-PUB-230606-35
- Date
- 2023
- Source
- Proceedings of the National Academy of Sciences of the United States of America 120: e2219649120e2219649120 (Journal)
- Registered Authors
- Lu, Fu-I
- Keywords
- Activin, Follistatin, Nodal, left–right asymmetry, patterning
- MeSH Terms
-
- Animals
- Body Patterning/genetics
- Follistatin/genetics
- Follistatin/metabolism
- Gene Expression Regulation, Developmental
- Transforming Growth Factor beta/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 37276408 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Fu, X.X., Zhuo, D.H., Zhang, Y.J., Li, Y.F., Liu, X., Xing, Y.Y., Huang, Y., Wang, Y.F., Cheng, T., Wang, D., Chen, S.H., Chen, Y.J., Jiang, G.N., Lu, F.I., Feng, Y., Huang, X., Ma, J., Liu, W., Bai, G., Xu, P.F. (2023) A spatiotemporal barrier formed by Follistatin is required for left-right patterning. Proceedings of the National Academy of Sciences of the United States of America. 120:e2219649120e2219649120.
Abstract
How left-right (LR) asymmetry emerges in a patterning field along the anterior-posterior axis remains an unresolved problem in developmental biology. Left-biased Nodal emanating from the LR organizer propagates from posterior to anterior (PA) and establishes the LR pattern of the whole embryo. However, little is known about the regulatory mechanism of the PA spread of Nodal and its asymmetric activation in the forebrain. Here, we identify bilaterally expressed Follistatin (Fst) as a regulator blocking the propagation of the zebrafish Nodal ortholog Southpaw (Spaw) in the right lateral plate mesoderm (LPM), and restricting Spaw transmission in the left LPM to facilitate the establishment of a robust LR asymmetric Nodal patterning. In addition, Fst inhibits the Activin-Nodal signaling pathway in the forebrain thus preventing Nodal activation prior to the arrival, at a later time, of Spaw emanating from the left LPM. This contributes to the orderly propagation of asymmetric Nodal activation along the PA axis. The LR regulation function of Fst is further confirmed in chick and frog embryos. Overall, our results suggest that a robust LR patterning emerges by counteracting a Fst barrier formed along the PA axis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping