FIGURE

FIGURE 8

ID

ZDB-FIG-230731-157

Publication

Farre et al., 2023 - Long wavelength-sensing cones of zebrafish retina exhibit multiple layers of transcriptional heterogeneity

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FIGURE 8

Expression of gngt2a in control (DMSO) and TH-treated (T3) larval zebrafish. (A–D) Projections of representative whole, imaged eyes. Note reduced expression domain of lws2 (cyan), and expanded expression domains of lws1 (magenta) and gngt2a (yellow) in T3-treated (B,D) vs. controls (A,C); expanded domains do not appear to align, however, (E–F’) Single z slices obtained from the same preparations. (G–G”’) Enlarged images of region within box in pane (F). (G) All imaging channels merged. (G’)gngt2a. (G”)lws1.(G”’)lws2.(H) qPCR quantification of gngt2a transcript abundance in pooled samples of whole larvae, n = 5 biological replicates per condition, p = 0.0967. (I) 3D fluorescence intensity quantification, n = 3 embryos per condition. (J) percent of eyes with expression of gngt2a in dorsal retina, n = 7 (DMSO), 5 (T3), p = 0.013. (proportion test). D, dorsal; T, temporal. *, p < 0.05; n.s., not significant.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.