FIGURE

Figure 2

ID

ZDB-FIG-230407-13

Publication

Cotellessa et al., 2023 - Defective Jagged1 signaling impacts GnRH development and contributes to congenital hypogonadotropic hypogonadism

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Figure 2

JAG1 and DLL1 proteins are expressed in GnRH neurons and in the olfactory/vomeronasal system of human fetuses.

(A–D) Representative confocal images of the nasal septum of a GW 9.5 fetus immunostained for GnRH1 (white), JAG1 (red), and TAG1 (green). JAG1 (arrows, B) is expressed by GnRH1-expressing neurons (arrows, A), as well as by other neurons belonging to the MM and by vomeronasal/terminal fibers expressing TAG1. (E–H) GnRH1 (white), DLL1 (red), and TAG1 (green) expression in a coronal section of the nasal region of a GW 9.5 fetus. DLL1 is expressed by GnRH neurons (arrows, E and F), by other neurons belonging to the MM, and by vomeronasal/terminal fibers. (I) Schematic representation of a GW 11.5 human fetus head (coronal view). The black box indicates the forebrain region of immunofluorescence analysis displayed in J–L. (J–L) DLL1 is expressed by a GnRH neuron that entered the forebrain. Scale bars: 20 μm. The experiments were replicated 3 times with similar results in n = 2 samples (GW 9.5 and GW 11.5). HF, human fetus.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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