FIGURE

Fig. 3

ID
ZDB-FIG-221226-241
Publication
Maradonna et al., 2022 - A zebrafish HCT116 xenograft model to predict anandamide outcomes on colorectal cancer
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Fig. 3

A HCT116 cells were directly injected into the Vein of Cuvier of 48-hpf WT larvae. The image shows the experimental procedure and the division of xenografts considering the number of HCT116 circulating cells 3 hpi. Image created with Biorender.com. B Stereomicroscope Representative Images of 3dpi Ctrl- (on the left) and AEA exposed xenografts (on the right). White arrows pinpoint HCT116 cells in metastasis. C, D Integrated density of HCT116 metastatic tumor cells in 3 dpi zebrafish larvae injected with small (C) and large (D) numbers of cells. The mean value of the integrated density in Ctrl was set as 100%. The error bar indicates SEM. (Small: Ctrl n = 48; AEA n = 50. Large: Ctrl n = 34; AEA n = 25 from three independent experiments). Statistical analysis was performed using Student’s t-test. ****p < 0.0001.

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Acknowledgments
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