FIGURE

Fig. 4

ID

ZDB-FIG-221128-35

Publication

Le et al., 2021 - Differential expression and hypoxia-mediated regulation of the N-myc downstream regulated gene family

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Fig. 4

Changes in ndrg transcript levels in response to hypoxia (3% oxygen). (A, B) Real-time qPCR analysis of 24 hpf zebrafish embryos exposed to 4 h (A, grey bars) or 8 h (B, white bars) of hypoxia relative to normoxic (stage-matched) controls (A, B, black bars) normalized to ef1a. (C) Plotted graphical summary of qPCR results. The y-axis in the graphs represents the relative normalized expression of each gene. All fold changes were derived using the formula, 2^−(ΔΔCT), error bars represent standard error of the mean. Significance was obtained using the unpaired, two-tailed t-test with Welch’s Correction. **p < .01. Reactions were run in triplicate with 7–8 biological replicates (n = 7–8).

Expression Data

Genes:	ndrg1a ndrg2 ndrg3a ndrg3b ndrg4
Fish:	AB
Condition:	hypoxia
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	hypoxia
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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