FIG 4 P. aeruginosa supernatants exhibit mild synergy with FLC compared to live Pseudomonas. P. aeruginosa and C. albicans were grown overnight in YPD media at 30°C. Overnight cultures supernatants were sterile filtered and added to 4 × 105 C. albicans in YPD liquid media along with 12.5 μg/mL of FLC. After 48 h of incubation at 30°C, cultures were 10-fold diluted and spotted onto YPD plates with antibiotics to count CFU. (A) Supernatant from PA14-dTom strain, (B) Supernatant from PA01 WT and ΔpvdFpchBA, (C) Supernatant from PA14 WT and Δphz, (D) Representative images of YPD plates showing the growth of C. albicans after 24 h of incubation. Data from 3 independent experiments. (p > 0.05 NS; < 0.05 *; <0.01 **; <0.001 ***; <0.0001 ****).
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