FIGURE

Fig. 3

ID

ZDB-FIG-220726-3

Publication

Hattab et al., 2022 - Pseudomonas Synergizes with Fluconazole against Candida during Treatment of Polymicrobial Infection

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Fig. 3

FIG 3 Iron supplementation partially reverses fungicidal effect in vitro, but phenazines and quorum sensing do not contribute to the effect. (A) FeCl₃ supplementation reverses P. aeruginosa-FLC synergy in vitro. Cocultures were performed with or without FLC treatment (12.5 μg/mL) and/or FeCl₃ (1 mM). Data from 3 independent experiments. (B) C. albicans growth after 48 h cocultures with P. aeruginosa WT or siderophore mutants: ΔpvdF, ΔpchB, ΔpvdFpchBA. Bar graph represents C. albicans growth in log₁₀ CFU/mL. Data is representative of 4 independent experiments and medians with interquartile ranges from three independent experiments are shown. (C) Cocultures of C. albicans with P. aeruginosa WT or ΔlasR mutant PA14 ΔlasR mutant is synergistic with FLC. Bar graph represents C. albicans growth in log₁₀ CFU/mL. Data is representative of 3 independent experiments. (D) Coculture of C. albicans with P. aeruginosa WT or phenazine deficient strains: PA14 Δphz, PA14 phzM::TnM, PA14 phzS::TnM in the presence or absence of FLC (12.5 μg/mL). Bar graph represents C. albicans growth in log₁₀ CFU/mL. Data is representative of 3 independent experiments. Data shown are the median with ranges. (p > 0.05 NS; < 0.05 *; <0.01 **; <0.001 ***; <0.0001 ****).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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