Figure 6

Early and mature osteoblasts respectively tracked by the fluorescence expression of Tg(sp7:mCherry) and Tg(bglap:EGFP). Four groups of treatment are ethanol at 0.1% (Control), VD group with VD3 at 10 pg/ml (VD), B at 10 ng/ml (B10), B at 10 ng/ml with VD3 at 10 pg/ml (B10VD). (A) An example of how the areas of the operculum and sp7⁺ and bglap⁺ were measured (big scale bar = 0.35 mm; small bar = 0.05 mm). (B) A table with merged pictures of operculum from the sp7:mCherry line stained with calcein and the bglap:EGFP line stained with AR-S (scale bar = 0.17 mm). (C) The sp7⁺ area inside the operculum was normalized with the total head area at time points 6, 9, 12, and 15 dpf. (D) The bglap⁺ area inside the operculum was normalized with the total head area at time points 6, 9, 12, and 15 dpf. One-way ANOVA and Tukey's post-hoc test were used. Data are presented as means ± SD, and different letters represent statistical significance at p < 0.05.