FIGURE

Figure 4

ID

ZDB-FIG-220518-114

Publication

Sun et al., 2022 - Notch-Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras^G12V-Induced Zebrafish Hepatocellular Carcinoma

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Figure 4

Hepatic Sox9 expression is upregulated after hepatocellular carcinoma induction, and the sox9b^fh313 mutant suppressed hepatocyte dedifferentiation. (A) Tg(fabp10a:Tet3G;TRE3G:kras^G12V-ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. (B,C) Sox9 antibody staining indicated the number of Sox9+ cells in the liver at 10 dpf in the DOX-induced control (n = 5), kras^G12V+ (n = 5) and kras^G12V+ and dnMAML+ (n = 5) groups with statistical results. (D) Tg(fabp10a:Tet3G;TRE3G:kras^G12V-ZsGreen1) treatment strategy in sox9b^fh313 mutant. (E) Three-dimensional images of Anxa4 antibody staining showed biliary duct changes in the DOX-induced kras^G12V+ and kras^G12V+ sox9b^fh313 mutants at 8 dpf and 10 dpf. (F) Monolayer images of Anxa4 antibody staining biliary duct changes in the DOX-induced kras^G12V+ and kras^G12V+ sox9b^fh313 mutant at 8 dpf and 10 dpf. Asterisks show significance: *—p < 0.05; **—p < 0.01. Scale bars—100 μm; error bars—S.D.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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