Fig. 3.

(A and B) RT-qPCR analysis of relative gpr126 and gpr112a mRNA expression (to the housekeeping gene rpl13a) in WT zebrafish and heterozygous and homozygous gpr126^bns341 (A) and gpr126^bns342 (B) zebrafish mutants at 48 hours postfertilization (hpf). Data are means ± SD (n = 1, 2, or 3 pools of three hearts per pool). (C to E) Lateral views of 100 hpf gpr126^+/+ (E), gpr126^{bns341/bns341} (D), and gpr126^{bns342/bns342} (E) zebrafish larvae. Inset panels: High-magnification views of the heart (boxed area). (F to I) Mid-sagittal confocal sections of gpr126^+/+ and gpr126^{bns342/bns342} zebrafish hearts at 80 hpf (F and G) and 96 hpf (H and I) in larvae derived from gpr126^bns342/+;Tg(kdrl:Hsa.HRAS-mCherry);Tg(myl7:EGFP-Hsa.HRAS) incrosses. (J and K) Mid-sagittal confocal sections of gpr126^+/+ and gpr126^{bns341/bns341} zebrafish hearts at 96 hpf in larvae derived from gpr126^bns341/+;Tg(myl7:EGFP-Hsa.HRAS) incrosses. Endocardial and myocardial membranes in (F) to (K) are labeled red and green, respectively. Right panels: High-magnification views of the outer curvature of the ventricular wall corresponding to the boxed areas. Arrowheads indicate emerging trabeculae. Scale bars, 25 μm. bv, bulboventricular valve; v, ventricle.