FIGURE

FIGURE 6

ID
ZDB-FIG-211207-6
Publication
Wang et al., 2021 - Identification of ACTA2 as a Key Contributor to Venous Malformation
Other Figures
All Figure Page
Back to All Figure Page
FIGURE 6

Effectiveness of acta2 knockdown was confirmed by RT-PCR and qRT-PCR. (A) The zebrafish acta2 gene was targeted by specific morpholino antisense to prevent proper splicing of exon 2 (E2I2-MO). (B) RT-PCR of acta2 transcript from control-MO and E2I2-MO morpholino-injected embryos 2 days after fertilization, demonstrating insertion of intron 2 and skipping of exon 2. (C,E) Sanger sequencing of both the wild type band and the intron 2-inserted band and the exon 2-skipped band validating the wild type sequence and the intron 2-inserted sequence (C) and the exon 2-skipped sequence (E). (D) Quantitative measurements of acta2 expression levels measured by qRT-PCR (***P < 0.0001). MO-targeted down-regulation of acta2. dpf, days post-fertilization.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol