ZFIN Image: Wang et al., 2021, FIGURE 6

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Figure Caption

FIGURE 6

Effectiveness of acta2 knockdown was confirmed by RT-PCR and qRT-PCR. (A) The zebrafish acta2 gene was targeted by specific morpholino antisense to prevent proper splicing of exon 2 (E2I2-MO). (B) RT-PCR of acta2 transcript from control-MO and E2I2-MO morpholino-injected embryos 2 days after fertilization, demonstrating insertion of intron 2 and skipping of exon 2. (C,E) Sanger sequencing of both the wild type band and the intron 2-inserted band and the exon 2-skipped band validating the wild type sequence and the intron 2-inserted sequence (C) and the exon 2-skipped sequence (E). (D) Quantitative measurements of acta2 expression levels measured by qRT-PCR (***P < 0.0001). MO-targeted down-regulation of acta2. dpf, days post-fertilization.

Acknowledgments

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