Figure 6—figure supplement 1

(a) Immunostaining for p-PXN^TYR118 and quantification of p-PXN^TYR118-positive focal points in EV-SkMel28, ∆MITF-X2, and ∆MITF-X6 cell lines. Error bars represents standard error of the mean, * p value <0.05 was calculated by one-way ANOVA (multiple correction with Dunnett test). (c) Co-expression of MITF mRNA and PXN expression in the 473 melanoma tumor samples displayed in the scatter plot with negative Pearson correlation coefficient. (d) Expression of MITF and PXN across 168 melanoma cell lines. Mutations are indicated for each cell line in colors (red:BRAF, orange:NRAS, yellow:cKIT, lime:double, green: WT, and blue: GNA11). (e) Negative correlation of MITF and PXN mRNA expression in 168 melanoma cell lines. (f–h) Immunostaining for p-PXN^TYR118 and MITF; Quantification of p-PXN^TYR118-positive focal points in miR-NTC and miR-MITF 501Mel and SkMel28 cells. (i) View of MITF ChIP-seq peaks in PXN from MITF CUT and RUN in SkMEL28 (rep1 and rep2), MITF ChIP-seq in COLO829 cells (Webster et al., 2014), and HA-MITF ChIP-seq in 501Mel (Laurette et al., 2015) loaded in IGV genome browser.