ZFIN ID: ZDB-FIG-200629-25
Chen et al., 2020 - Capn3 depletion causes Chk1 and Wee1 accumulation and disrupts synchronization of cell cycle reentry during liver regeneration after partial hepatectomy. Cell regeneration (London, England)   9:8 Full text @ Cell Regen (Lond)
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Protruding-mouth to Adult

Fig. 9

Hepatic accumulation of Chk1 and Wee1 in capn3bΔ19Δ14 at 3dpH. a Elevation of p53 protein level in capn3bΔ19Δ14 after PH. Western blot analysis of p53 at different time point after PH as indicated. Total proteins were extracted from the liver tissues of sham, 3dpH, 5dpH, 7dpH, 10dpH and 14dpH, respectively. GAPDH, loading control. b Western blot of Capn3b and Chk1 in WT and capn3bΔ19Δ14 mutant embryos at 3.5dpf and 5dpf grown at 30 °C and 34.5 °C, respectively. The embryos were shifted to 34.5 °C at 12hpf till the time of harvesting. β-Actin: loading control. c Western blot of Chk1 in WT and capn3bΔ19Δ14 sham and PH groups at 3dpH group. Fibrillarin: loading control. d Co-immunostaining of Wee1 and Fibrillarin in the liver of WT and capn3bΔ19Δ14 mutant fish at 3dpH. DAPI: staining nuclei

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
capn3b Ab1-capn3b capn3bzju20/zju20(AB) heat exposure Protruding-mouth Not Detected whole organism WB
Ab1-capn3b Day 5 Not Detected whole organism WB
tp53 capn3bzju20/zju20(AB) amputation: liver Adult liver regenerating tissue WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-wee1 IHC AB amputation: liver Adult liver nucleolus
IHC Adult liver nucleus
IHC capn3bzju20/zju20(AB) amputation: liver Adult liver nucleolus
IHC Adult liver nucleus
Ab5-chek1 WB AB amputation: liver Adult liver
WB Adult liver regenerating tissue
WB capn3bzju20/zju20(AB) heat exposure Protruding-mouth whole organism
WB Day 5 whole organism
WB capn3bzju20/zju20(AB) amputation: liver Adult liver
WB Adult liver regenerating tissue
Phenotype Details
Fish Conditions Stage Phenotype
AB amputation: liver Adult liver Ab5-chek1 labeling increased amount, abnormal
capn3bzju20/zju20(AB) heat exposure Protruding-mouth whole organism capn3b expression absent, abnormal
Protruding-mouth whole organism Ab5-chek1 labeling increased amount, abnormal
Day 5 whole organism capn3b expression absent, abnormal
Day 5 whole organism Ab5-chek1 labeling increased amount, abnormal
capn3bzju20/zju20(AB) amputation: liver Adult liver Ab5-chek1 labeling increased amount, abnormal
Adult liver nucleolus Ab1-wee1 labeling increased amount, abnormal
Adult liver nucleus Ab1-wee1 labeling decreased amount, abnormal
Adult liver regenerating tissue tp53 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell regeneration (London, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Regen (Lond)