ZFIN ID: ZDB-FIG-200629-19
Chen et al., 2020 - Capn3 depletion causes Chk1 and Wee1 accumulation and disrupts synchronization of cell cycle reentry during liver regeneration after partial hepatectomy. Cell regeneration (London, England)   9:8 Full text @ Cell Regen (Lond)
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Long-pec to Adult

Fig. 2

Defective development of capn3b mutant zebrafish under environment stress. a, b WISH using the fabp10a probe on 3dpf- and 5dpf-old WT and capn3bΔ19Δ14 embryos grown at the low (60 embryo/dish) (left panel) and high (120cm2/dish) (right panel) rearing density in a 9 cm-diameter dish (a). In (b), the data of liver sizes were compared between 3dpf- and 5dpf-old WT or between 3dpf- and 5dpf-old capn3bΔ19Δ14 mutant embryos under low (L) and high (H) rearing intensity. c WISH using the fabp10a and trypsin probes on WT and capn3bΔ19Δ14 embryos at 2dpf and 3dpf. Embryos were shifted to 34.5 °C at 12 hpf till the time of sample harvesting. Numerator/denominator: number of embryos displayed the shown phenotype over total number of genotyped embryos. d Photo images showing the curved body phenotype displayed by the 3dpf-old capn3bΔ19Δ14 mutant but not WT embryos grown at 34.5 °C. e Body lengths of 6-, 8- and 12-months-old WT and capn3bΔ19Δ14 fish. f LBR of 6- and 12-months-old WT and capn3bΔ19Δ14 fish. Student’s T-test for statistical analyses, *, p < 0.05; ***, p < 0.001; ****, p < 0.0001

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp10a AB heat exposure Long-pec liver ISH
Protruding-mouth liver ISH
capn3bzju20/zju20(AB) heat exposure Long-pec Not Detected liver ISH
Protruding-mouth liver ISH
prss1 AB heat exposure Long-pec exocrine pancreas ISH
Protruding-mouth exocrine pancreas ISH
capn3bzju20/zju20(AB) heat exposure Long-pec Not Detected exocrine pancreas ISH
Protruding-mouth exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
capn3bzju20/zju20(AB) standard conditions Adult growth delayed, abnormal
Adult whole organism decreased size, abnormal
capn3bzju20/zju20(AB) heat exposure Long-pec embryonic liver development delayed, abnormal
Long-pec exocrine pancreas prss1 expression absent, abnormal
Long-pec exocrine pancreas development delayed, abnormal
Long-pec liver fabp10a expression absent, abnormal
Protruding-mouth whole organism curved, abnormal
capn3bzju20/zju20(AB) overcrowding Protruding-mouth liver increased size, abnormal
Day 5 liver increased size, abnormal
capn3bzju20/zju20(AB) undercrowding Protruding-mouth liver decreased size, abnormal
Day 5 liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell regeneration (London, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Regen (Lond)