ZFIN ID: ZDB-FIG-200629-24
Chen et al., 2020 - Capn3 depletion causes Chk1 and Wee1 accumulation and disrupts synchronization of cell cycle reentry during liver regeneration after partial hepatectomy. Cell regeneration (London, England)   9:8 Full text @ Cell Regen (Lond)
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 8

Chk1 and Wee1 are substrates of the Def-Capn3b complex. a Diagram showing the Capn3 recognition motif (left panel) and the locations of this motif in zebrafish, human and mouse Chk1 (middle panel) and Wee1 (right panel) proteins. b, c Western blot of Def, HA-p53R143H, HA-Chk1 and HA-Wee1S44A in WT embryos injected with HA-p53R143H, HA-Chk1 or HA-Wee1S44A mRNA combined with or without def mRNA (b). Western blot of HA-Chk1 and HA-Chk1Δ in WT embryos injected with HA-Chek1or HA-Chek1Δ mRNA combined with or without def mRNA (c). Total proteins were extracted at 8-h post-injection. β-Actin: loading control. d Western blot of the endogenous Chk1 and Wee1 proteins in WT and def−/− mutant embryos at 5dpf. Tubulin: loading control. e, f Co-immunostaining of Chk1 (e) or Wee1 (F) with Fibrillarin in the liver of WT with def−/− at 5dpf. DAPI: stain nuclei

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-fbl IHC AB standard conditions Day 5 liver nucleolus
IHC utp25hi429Tg/hi429Tg standard conditions Day 5 liver nucleolus
Ab1-wee1 IHC AB standard conditions Day 5 liver nucleolus
IHC Day 5 liver nucleus
IHC utp25hi429Tg/hi429Tg standard conditions Day 5 liver nucleolus
IHC Day 5 liver nucleus
WB Day 5 whole organism
Ab5-chek1 IHC AB standard conditions Day 5 liver nucleolus
IHC Day 5 liver nucleus
IHC utp25hi429Tg/hi429Tg standard conditions Day 5 liver nucleolus
IHC Day 5 liver nucleus
WB Day 5 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
utp25hi429Tg/hi429Tg standard conditions Day 5 liver nucleolus Ab1-wee1 labeling increased amount, abnormal
Day 5 liver nucleolus Ab5-chek1 labeling increased amount, abnormal
Day 5 liver nucleus Ab1-wee1 labeling decreased amount, abnormal
Day 5 liver nucleus Ab5-chek1 labeling decreased amount, abnormal
Day 5 whole organism Ab1-wee1 labeling increased amount, abnormal
Day 5 whole organism Ab5-chek1 labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell regeneration (London, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Regen (Lond)