Phenotypic rescue experiments using demethylase-deficient human LSD1 mutant. (A) The gata1a expression at 15 hpf in the lateral plate mesoderm (arrowheads) of wild-type or kdm1ait627 embryos injected with or without mRNAs encoding indicated wild-type or K661A mutant-type human LSD1 was detected by WISH analysis. (B) Immunoblot analysis using anti-FLAG antibody of FLAG-tagged LSD1 proteins in 15-hpf wild-type embryos. Total protein loading was shown by CBB staining. (C) Expression of alas2 and hbbe1.1at 20 hpf in the intermediate cell mass (arrowheads). (D) Expression of etv2 at 15 hpf in the lateral plate mesoderm (arrowheads).