ZFIN ID: ZDB-FIG-200528-2
Tamaoki et al., 2020 - Splicing- and demethylase-independent functions of LSD1 in zebrafish primitive hematopoiesis. Scientific Reports   10:8521 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 10-13 somites to 20-25 somites

Fig. 4

Phenotypic rescue experiments using demethylase-deficient human LSD1 mutant. (A) The gata1a expression at 15 hpf in the lateral plate mesoderm (arrowheads) of wild-type or kdm1ait627 embryos injected with or without mRNAs encoding indicated wild-type or K661A mutant-type human LSD1 was detected by WISH analysis. (B) Immunoblot analysis using anti-FLAG antibody of FLAG-tagged LSD1 proteins in 15-hpf wild-type embryos. Total protein loading was shown by CBB staining. (C) Expression of alas2 and hbbe1.1at 20 hpf in the intermediate cell mass (arrowheads). (D) Expression of etv2 at 15 hpf in the lateral plate mesoderm (arrowheads).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alas2 AB standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
kdm1ait627/it627 (AB) standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
etsrp AB standard conditions 10-13 somites lateral plate mesoderm ISH
kdm1ait627/it627 (AB) standard conditions 10-13 somites lateral plate mesoderm ISH
hbbe1.1 AB standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
kdm1ait627/it627 (AB) standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kdm1ait627/it627 (AB) standard conditions 10-13 somites lateral plate mesoderm etsrp expression increased distribution, abnormal
20-25 somites intermediate cell mass of mesoderm alas2 expression decreased distribution, abnormal
20-25 somites intermediate cell mass of mesoderm hbbe1.1 expression decreased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.