ZFIN ID: ZDB-FIG-200528-1
Tamaoki et al., 2020 - Splicing- and demethylase-independent functions of LSD1 in zebrafish primitive hematopoiesis. Scientific Reports   10:8521 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 5-9 somites

Fig. 3

Phenotypic rescue experiments using human and zebrafish LSD1 variants. (A) The gata1a expression at 15 hpf in the lateral plate mesoderm (arrowheads) of wild-type or kdm1ait627 embryos injected with or without mRNAs encoding indicated human LSD1 variants was detected by WISH analysis. +2a, LSD1+2a; +8a, LSD1+8a; +2a8a, LSD1+2a8a. The numbers in each picture indicate the numbers of embryos with similar staining profiles and tested embryos. WISH was performed in at least two separated trials. (B) Immunoblot analysis using anti-FLAG antibody of FLAG-tagged LSD1 variants in 15-hpf wild-type embryos injected with or without mRNAs encoding indicated human LSD1 variants. Total protein loading was shown by CBB staining. (C) The gata1a expression at 15 hpf in the lateral plate mesoderm (arrowheads) of wild-type or kdm1ait627 embryos injected with or without mRNAs encoding indicated zebrafish LSD1 variants. +2al, LSD1+2al; +8al, LSD1+8al; +2a8al, LSD1+2a8a. (D) Immunoblot analysis using anti-FLAG antibody of FLAG-tagged LSD1 variants in 15-hpf wild-type embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata1a AB standard conditions 5-9 somites lateral plate mesoderm ISH
kdm1ait627/it627 (AB) standard conditions 5-9 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kdm1ait627/it627 (AB) standard conditions 5-9 somites lateral plate mesoderm gata1a expression decreased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.