ZFIN ID: ZDB-FIG-200421-37
Adusumilli et al., 2020 - miR-7 Controls the Dopaminergic/Oligodendroglial Fate through Wnt/β-catenin Signaling Regulation. Cells   9(3) Full text @ Cells
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Figure 6

miR-7a negatively regulates Wnt signaling and diencephalic th-positive cells in the zebrafish brain (AG’’): The knock-down of miR-7a activity by two independent morpholinos, targeting the immature and mature forms (loop7a1/2/3 MO, shown in E,E’,E’’, and mir7a MO, shown in F,F’,F’’) increases the amount of th-positive brain cells (in green) and the activity of a Wnt reporter (in red), compared with controls not injected (A,A’,A’’), co-injected with non-functional morpholino (mismMO) + non-functional miR-7a (NCDP) (B,B’,B’’), or co-injected (rescued) either with loop7a1/2/3 MO + 7DP (C,C’,C’’) or with miR-7a MO + 7DP (D,D’,D’’). On the contrary, over-expression of 7DP reduces the amount of th-positive brain cells and the activity of the Wnt reporter (G,G’,G’’). All pictures show the head region of 40 hpf embryos in lateral view, with the anterior to the left. te: telencephalon; di: diencephalon; hi: hindbrain. (H,I): Charts showing the th-positive cell counting (H), and the measure of Wnt reporter activity (I), expressed as fluorescence relative intensity (R.I.), in the seven conditions. Sample size n = 5 measures/condition; (*) p < 0.05; (**) p < 0.01; (***) p < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ia4Tg standard conditions Prim-25 brain IHC
ia4Tg + MO1-mir7a standard conditions Prim-25 brain IHC
ia4Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3 standard conditions Prim-25 brain IHC
th ia4Tg control Prim-25 brain ISH
ia4Tg + MO1-mir7a standard conditions Prim-25 brain ISH
ia4Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3 standard conditions Prim-25 brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ia4Tg + MO1-mir7a standard conditions Prim-25 brain th expression increased amount, abnormal
Prim-25 brain GFP expression increased amount, abnormal
ia4Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3 standard conditions Prim-25 brain th expression increased amount, abnormal
Prim-25 brain GFP expression increased amount, abnormal
Acknowledgments:
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