ZFIN ID: ZDB-FIG-200421-39
Adusumilli et al., 2020 - miR-7 Controls the Dopaminergic/Oligodendroglial Fate through Wnt/β-catenin Signaling Regulation. Cells   9(3) Full text @ Cells
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Pec-fin

Figure 8

miR-7 positively regulates Shh signaling responsiveness and olig2+ cells in the zebrafish diencephalon. (AD) Shh:GFP reporter embryos, injected with NCDP and mismatch morpholino, were used as controls (A). Injection of miR-7 7DP (B) or miR-7a morpholino alone (D) elicits opposite effects on Shh-responsive cells located in the diencephalic region, between the telencephalic-diencephalic boundary (TDB), the diencephalic-mesencephalic boundary (DMB), and the hypothalamus (H), compared with injected controls (A). Co-injection of 7DP and miR-7a MO rescues both conditions (C). All panels show the head region of 60 hpf embryos in lateral view, with the anterior to the left. (E) Chart refers to A–D experimental series. Error bars represent SEM; the experiments were repeated thrice. Sample size n = 5 measures/condition for quantification using Volocity software; (***) p < 0.001. (FI) olig2:EGFP embryos, injected with NCDP and mismatch morpholino, were used as controls (F). Telencephalic (te), diencephalic (di) and hindbrain (hi) expression of olig2-dependent EGFP appears increased in embryos overexpressing miR-7 7DP (G), and reduced in miR-7a morphants (I). Co-injection of miR-7a MO and 7DP rescues the phenotype (H). All panels are lateral views of the head region at 40 hpf, with the anterior to the left. (J) Chart refers to F–I experimental series. Error bars represent SEM; the experiments were repeated thrice. Sample size n = 5 measures/condition for quantification using Volocity software; (*) p < 0.05; (**) p < 0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP vu12Tg control Prim-25 diencephalon IFL
Prim-25 hindbrain IFL
Prim-25 telencephalon IFL
vu12Tg + MO1-mir7a standard conditions Prim-25 diencephalon IFL
Prim-25 hindbrain IFL
Prim-25 telencephalon IFL
vu12Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3 standard conditions Prim-25 diencephalon IFL
Prim-25 hindbrain IFL
Prim-25 telencephalon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nu15Tg; y1Tg + MO1-mir7a standard conditions Pec-fin brain smoothened signaling pathway decreased occurrence, abnormal
Pec-fin forebrain midbrain boundary smoothened signaling pathway decreased occurrence, abnormal
Pec-fin hypothalamus smoothened signaling pathway decreased occurrence, abnormal
Pec-fin telencephalon diencephalon boundary smoothened signaling pathway decreased occurrence, abnormal
nu15Tg; y1Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3 standard conditions Pec-fin brain smoothened signaling pathway decreased occurrence, abnormal
Pec-fin forebrain midbrain boundary smoothened signaling pathway decreased occurrence, abnormal
Pec-fin hypothalamus smoothened signaling pathway decreased occurrence, abnormal
Pec-fin telencephalon diencephalon boundary smoothened signaling pathway decreased occurrence, abnormal
vu12Tg + MO1-mir7a standard conditions Prim-25 diencephalon EGFP expression decreased amount, abnormal
Prim-25 hindbrain EGFP expression decreased amount, abnormal
Prim-25 telencephalon EGFP expression decreased amount, abnormal
vu12Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3 standard conditions Prim-25 diencephalon EGFP expression decreased amount, abnormal
Prim-25 hindbrain EGFP expression decreased amount, abnormal
Prim-25 telencephalon EGFP expression decreased amount, abnormal
Acknowledgments:
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