ZFIN ID: ZDB-FIG-200325-202
Bagwell et al., 2020 - Notochord vacuoles absorb compressive bone growth during zebrafish spine formation. eLIFE   9 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Days 14-20
PHENOTYPE:
Fish:
Observed In:
Stage: Days 14-20

Fig. 8

Nuclear distribution and shape indicate that hydrostatic pressure of the notochord is decreased in spzl mutants.

( A–B) Confocal images of cross sections of 14 dpf WT and spzl-/- larvae section. Vacuolated cells are labeled with SAG:gal4;UAS:GFP and nuclei are stained with DAPI. Scale bar = 50 µm ( C) Plot of vacuolated cell nuclear distribution for WT (red, n = 29) and spzl-/- (black, n = 32). ( D) Nuclear volume in WT and spzl-/- at 14 dpf. ( E–F) Reconstructions of nuclei in WT ( E) and spzl-/- ( F) and two different viewing angles. Scale bar = 20 µm ( G) Sphericity of WT and spzl-/- nuclei at 14 dpf. p-values were determined by an un-paired t-test using Welch’s correction.











Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP nksagff214aGt ; nkuasgfp1aTg standard conditions Days 14-20 notochord inner cell cytoplasm IFL
Days 14-20 Not Detected notochord inner cell vacuolar lumen IFL
nksagff214aGt ; nkuasgfp1aTg ; dstyks739/s739 standard conditions Days 14-20 notochord inner cell cytoplasm IFL
Days 14-20 Not Detected notochord inner cell vacuolar lumen IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nksagff214aGt ; nkuasgfp1aTg ; dstyks739/s739 standard conditions Days 14-20 notochord inner cell nucleus mislocalised medially, abnormal
Days 14-20 notochord inner cell nucleus normal volume, normal
Days 14-20 notochord inner cell nucleus spheroid, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife