ZFIN Figure: Bagwell et al., 2020, Fig. 5

ZFIN ID: ZDB-FIG-200325-197

ZFIN ID: ZDB-FIG-200325-197

Bagwell et al., 2020 - Notochord vacuoles absorb compressive bone growth during zebrafish spine formation. eLIFE 9 Full text @ Elife

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	pd1083Tg ; mu286Tg pd1083Tg ; dstyk^s739/s739 ; mu286Tg
Anatomical Term:	notochord inner cell vacuolar membrane
Stage Range:	Long-pec to Pec-fin

PHENOTYPE:

Fish:	pd1083Tg ; dstyk^s739/s739 ; mu286Tg
Observed In:	notochord inner cell notochord cell vacuolation notochord inner cell vesicle fusion with vacuole
Stage Range:	Long-pec to Pec-fin

Fig. 5

Dstyk regulates fusion of pre-vacuolar carriers with the notochord vacuole.

( A–B) Maximum projection of a light sheet microscopy live image of WT and spzl-/- embryos at 48hpf and 65hpf, respectively. ( C–D) Still images from a 17 hr light sheet movie of a WT and spzl-/-embryos zebrafish embryo during vacuole biogenesis depicting vacuole fusion events. Vacuole membranes are labeled by NFATC:gal4;UAS:GFPrab32 expression. Arrows point to GFP-Rab32 positive vesicles. Arrowheads point to large vesicle (vacuolinos) docking events. n = 2 animals per genotype. Asterisks mark fusion events. Scale bar = 100 µm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	pd1083Tg ; dstyk^s739/s739 ; mu286Tg	standard conditions	Long-pec	notochord inner cell vacuolar membrane	IFL
			Pec-fin	notochord inner cell vacuolar membrane	IFL
	pd1083Tg ; mu286Tg	standard conditions	Long-pec	notochord inner cell vacuolar membrane	IFL
			Pec-fin	notochord inner cell vacuolar membrane	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
pd1083Tg ; dstyk^s739/s739 ; mu286Tg	standard conditions	Long-pec	notochord inner cell notochord cell vacuolation decreased process quality, abnormal
		Long-pec	notochord inner cell vesicle fusion with vacuole decreased occurrence, abnormal
		Pec-fin	notochord inner cell notochord cell vacuolation decreased process quality, abnormal
		Pec-fin	notochord inner cell vesicle fusion with vacuole decreased occurrence, abnormal

Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife