ZFIN ID: ZDB-FIG-200325-197 |
Bagwell et al., 2020
- Notochord vacuoles absorb compressive bone growth during zebrafish spine formation.
eLIFE
9
Full text @ Elife
Fig. 5 Dstyk regulates fusion of pre-vacuolar carriers with the notochord vacuole.( A–B) Maximum projection of a light sheet microscopy live image of WT and spzl-/- embryos at 48hpf and 65hpf, respectively. ( C–D) Still images from a 17 hr light sheet movie of a WT and spzl-/-embryos zebrafish embryo during vacuole biogenesis depicting vacuole fusion events. Vacuole membranes are labeled by NFATC:gal4;UAS:GFPrab32 expression. Arrows point to GFP-Rab32 positive vesicles. Arrowheads point to large vesicle (vacuolinos) docking events. n = 2 animals per genotype. Asterisks mark fusion events. Scale bar = 100 µm. |
Gene Expression Details
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
EGFP | pd1083Tg ; dstyks739/s739 ; mu286Tg | standard conditions | Long-pec | notochord inner cell vacuolar membrane | IFL | |
Pec-fin | notochord inner cell vacuolar membrane | IFL | ||||
pd1083Tg ; mu286Tg | standard conditions | Long-pec | notochord inner cell vacuolar membrane | IFL | ||
Pec-fin | notochord inner cell vacuolar membrane | IFL |
Antibody Labeling Details
No data available
Phenotype Details
Acknowledgments: | ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife |
---|