ZFIN ID: ZDB-FIG-200325-197
Bagwell et al., 2020 - Notochord vacuoles absorb compressive bone growth during zebrafish spine formation. eLIFE   9 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 5

Dstyk regulates fusion of pre-vacuolar carriers with the notochord vacuole.

( A–B) Maximum projection of a light sheet microscopy live image of WT and spzl-/- embryos at 48hpf and 65hpf, respectively. ( C–D) Still images from a 17 hr light sheet movie of a WT and spzl-/-embryos zebrafish embryo during vacuole biogenesis depicting vacuole fusion events. Vacuole membranes are labeled by NFATC:gal4;UAS:GFPrab32 expression. Arrows point to GFP-Rab32 positive vesicles. Arrowheads point to large vesicle (vacuolinos) docking events. n = 2 animals per genotype. Asterisks mark fusion events. Scale bar = 100 µm.






Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pd1083Tg ; dstyks739/s739 ; mu286Tg standard conditions Long-pec notochord inner cell vacuolar membrane IFL
Pec-fin notochord inner cell vacuolar membrane IFL
pd1083Tg ; mu286Tg standard conditions Long-pec notochord inner cell vacuolar membrane IFL
Pec-fin notochord inner cell vacuolar membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pd1083Tg ; dstyks739/s739 ; mu286Tg standard conditions Long-pec notochord inner cell notochord cell vacuolation decreased process quality, abnormal
Long-pec notochord inner cell vesicle fusion with vacuole decreased occurrence, abnormal
Pec-fin notochord inner cell notochord cell vacuolation decreased process quality, abnormal
Pec-fin notochord inner cell vesicle fusion with vacuole decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife