ZFIN ID: ZDB-FIG-200325-194
Bagwell et al., 2020 - Notochord vacuoles absorb compressive bone growth during zebrafish spine formation. eLIFE   9 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 3

Loss of Dstyk function causes the spzl phenotype.

( A) Genome view of SNPtrack linkage analysis of exome sequencing data. The spzl locus maps to chromosome 22 (top panel), with a high probability interval (red line) centered around 410–570 Kbps. Recombination events with a panel of 83 mutants at either side of the spzl locus define a ~ 1.2 Mb critical interval. ( B) Genome organization of the critical genomic interval near the spzl locus. A nonsense mutation generates an early stop codon in dstyk. ( C) Cartoon depicting predicted gene product structure of dstyk-202, spzls739 and the Crispr/Cas9 generated dstykpd1133 allele. ( D) Dstyk is a Ser/Thr and Tyr kinase. Heat map depicting amino acid similarity between the human and zebrafish proteins. The kinase domain (highlighted) is 93.73% similar. ( E) In situ hybridization for dstyk expression at the 1 cell stage, 10 somites, 20 somites, and 24 hpf, respectively. Arrows point to highly expressing areas of the notochord. ( F–H) Bright field images of spzl-/-and WT sibling, spzl;dstykpd1133-/- and WT sibling, dstykpd1133 homozygous mutant and WT sibling, scale bars = 1 mm. DIC images of the notochord of 48 hpf embryos. Arrows point to fragmented vacuoles. Scale bars are 100 µm.





Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dstyk AB/TL standard conditions 1-cell whole organism ISH
10-13 somites notochord ISH
20-25 somites notochord posterior region ISH
Prim-5 notochord posterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dstykpd1133/+ ; dstyks739/+ standard conditions Long-pec notochord decreased length, abnormal
Long-pec notochord inner cell notochord cell vacuolation decreased process quality, abnormal
Long-pec notochord inner cell vacuole broken, abnormal
dstykpd1133/pd1133 standard conditions Long-pec notochord decreased length, abnormal
Long-pec notochord inner cell notochord cell vacuolation decreased process quality, abnormal
Long-pec notochord inner cell vacuole broken, abnormal
dstyks739/s739 standard conditions Long-pec notochord decreased length, abnormal
Long-pec notochord inner cell notochord cell vacuolation decreased process quality, abnormal
Long-pec notochord inner cell vacuole broken, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife