ZFIN ID: ZDB-FIG-200314-19
Grzegorski et al., 2020 - Disruption of the kringle 1 domain of prothrombin leads to late onset mortality in zebrafish. Scientific Reports   10:4049 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Days 7-13

Figure 3

Genome editing creates a 14 bp genomic deletion with a resulting decrease in mRNA expression. (A) Alignment of Sanger sequencing with the chromosome 7 genomic region showed an overall 17 bp genomic deletion replaced with a 3 bp insertion; outlined in red, resulting in a net 14 bp deletion. (B) in situ hybridization demonstrated reduction of transcript at 72 and 120 hours post fertilization in homozygous mutants compared to control siblings. Spatial regulation remained intact with expression restricted primarily to the liver. (C) qPCR data of f2 expression reveals significant decrease of 45% in the homozygous mutant embryos. (D) Semi-quantitative RT-PCR of embryos shows a mutant band ~30 bp smaller than expected (later shown to be a 45 bp deletion, Fig. 4). Quantitation of the bands reveal that the mutant band is only 26% of the total in heterozygotes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
f2 AB/TL standard conditions Protruding-mouth liver ISH
Day 5 liver ISH
Days 7-13 whole organism RTPCR
f2mi4008/mi4008(AB/TL) standard conditions Protruding-mouth liver ISH
Day 5 liver ISH
Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f2mi4008/mi4008(AB/TL) standard conditions Protruding-mouth liver f2 expression decreased amount, abnormal
Day 5 liver f2 expression decreased amount, abnormal
Days 7-13 whole organism f2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.