ZFIN ID: ZDB-FIG-200314-23
Grzegorski et al., 2020 - Disruption of the kringle 1 domain of prothrombin leads to late onset mortality in zebrafish. Scientific Reports   10:4049 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Protruding-mouth to Day 6

Figure 6

Loss of thrombin activity results in defects in secondary hemostasis. (A) Larvae were immobilized in agarose, subjected to laser-mediated endothelial injury (green arrow) of the venous (PCV, blue) or arterial (dorsal aorta, red) circulation, and followed for 2 minutes by a blinded observer. (B) Genetic ablation of f2 resulted in the inability to form induced PCV thrombi at 3 dpf and was not influenced by inhibiting fibrinolysis (ɛ-aminocaproic acid treatment, blue). (C) Overexpression of human F2 cDNA (blue) rescued the ability to form thrombi in the PCV at 3 dpf. (D) Homozygous mutant larvae demonstrated a significant impairment in arterial thrombus formation at 5 and 6 dpf without any changes in the time to initial thrombocyte attachment (E). (F) The number of thrombocytes attached to the site of injury in 2 minutes was significantly increased at 6 dpf in f2 homozygous mutants. Statistical significance assessed by Mann-Whitney U testing.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f2mi4008/mi4008 (AB/TL) light damage: posterior cardinal vein, chemical treatment by environment: 6-aminohexanoic acid Protruding-mouth blood coagulation process quality, abnormal
Protruding-mouth posterior cardinal vein blood coagulation, fibrin clot formation delayed, abnormal
f2mi4008/mi4008 (AB/TL) light damage: posterior cardinal vein Protruding-mouth blood coagulation process quality, abnormal
Protruding-mouth posterior cardinal vein blood coagulation, fibrin clot formation delayed, abnormal
f2mi4008/mi4008; la2Tg (AB/TL) light damage: dorsal aorta Day 5 blood coagulation process quality, abnormal
Day 5 dorsal aorta blood coagulation, fibrin clot formation delayed, abnormal
Day 6 blood coagulation process quality, abnormal
Day 6 dorsal aorta blood coagulation, fibrin clot formation delayed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.