ZFIN ID: ZDB-FIG-200208-6
Erickson et al., 2020 - The lhfpl5 Ohnologs lhfpl5a and lhfpl5b Are Required for Mechanotransduction in Distinct Populations of Sensory Hair Cells in Zebrafish. Frontiers in molecular neuroscience   12:320 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

FIGURE 6

Lhfpl5a requires MET complex proteins Pcdh15a, Cdh23, and Myo7a for normal localization in the stereocilia of zebrafish hair cells. (A,B) Immunostain of Pcdh15a (magenta) in the lateral cristae of wild type and lhfpl5atm290d mutants at 3 dpf. Phalloidin-stained actin of the hair bundle is shown in green. Arrows indicate areas of Pcdh15a accumulation. (C–F’) Representative images of GFP-Lhfpl5a (vo23Tg) in the lateral cristae hair bundles of wild type (C,C’) and pcdh15apsi7(D,D’), cdh23nl9(E,E’), and myo7aaty220(F,F’) mutants. White arrows indicate GFP signal in the presumptive kinocilial linkages, yellow arrow heads indicate GFP signal in the stereocilia or the base of the hair bundle, and brackets indicate GFP signal in the kinocilium. Scale bars = 3 μm in (A,B); 2 μm in (C–F’).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP cdh23nl9/nl9; vo23Tg standard conditions Protruding-mouth lateral crista kinocilium IFL
Protruding-mouth lateral crista stereocilium IFL
myo7aaty220/ty220; vo23Tg standard conditions Protruding-mouth Not Detected lateral crista kinocilium IFL
Protruding-mouth lateral crista stereocilium IFL
pcdh15apsi7/psi7; vo23Tg standard conditions Protruding-mouth Not Detected lateral crista kinocilium IFL
Protruding-mouth Not Detected lateral crista stereocilium shaft IFL
Protruding-mouth lateral crista stereocilium tip IFL
vo23Tg standard conditions Protruding-mouth lateral crista kinocilium IFL
Protruding-mouth lateral crista stereocilium shaft IFL
Protruding-mouth lateral crista stereocilium tip IFL
pcdh15a Ab2-pcdh15a WT standard conditions Protruding-mouth lateral crista stereocilium shaft IHC
Ab2-pcdh15a Protruding-mouth lateral crista stereocilium tip IHC
Ab2-pcdh15a lhfpl5atm290d/tm290d standard conditions Protruding-mouth Not Detected lateral crista stereocilium shaft IHC
Ab2-pcdh15a Protruding-mouth lateral crista stereocilium tip IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cdh23nl9/nl9; vo23Tg standard conditions Protruding-mouth lateral crista kinocilium EGFP expression increased amount, abnormal
Protruding-mouth lateral crista stereocilium EGFP expression decreased amount, abnormal
lhfpl5atm290d/tm290d standard conditions Protruding-mouth lateral crista stereocilium bundle splayed, abnormal
Protruding-mouth lateral crista stereocilium shaft pcdh15a expression absent, abnormal
Protruding-mouth lateral crista stereocilium tip pcdh15a expression decreased amount, abnormal
myo7aaty220/ty220; vo23Tg standard conditions Protruding-mouth lateral crista kinocilium EGFP expression absent, abnormal
Protruding-mouth lateral crista stereocilium EGFP expression decreased amount, abnormal
pcdh15apsi7/psi7; vo23Tg standard conditions Protruding-mouth lateral crista kinocilium EGFP expression absent, abnormal
Protruding-mouth lateral crista stereocilium bundle splayed, abnormal
Protruding-mouth lateral crista stereocilium shaft EGFP expression absent, abnormal
Protruding-mouth lateral crista stereocilium tip EGFP expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in molecular neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Mol. Neurosci.