FIGURE

Figure 6

ID

ZDB-FIG-191230-1736

Publication

Simbulan-Rosenthal et al., 2019 - CRISPR-Cas9 Knockdown and Induced Expression of CD133 Reveal Essential Roles in Melanoma Invasion and Metastasis

Other Figures

All Figure Page

Back to All Figure Page

Figure 6

Dox-inducible CD133 expression in BAK-P cells as verified by qPCR (a) and immunoblot analysis (b). (c) Dox-inducible CD133 expression significantly increases cell invasion in transwell assays. (d) Immunoblot analysis with antibodies to CD133, MMP9, MMP2, or β-Actin for loading controls, show increased levels of CD133 as well as MP2 and MMP9 secreted by Dox-induced cells. (e) Zebrafish assays reveal enhanced metastasis in Dox-induced cells. (f,g left panels) Invasion assay results with other melanoma cell lines POT (f) and SK-MEL2 (g) CD133 CRISPR-cas9 SC versus T3 lines are consistent with results with BAK-P cells, showing that knockdown of CD133 (f,g right panels) results in decreased cell invasion. *, **, *** represent p < 0.05 and p < 0.01, and p < 0.001, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers