ZFIN Image: Simbulan-Rosenthal et al., 2019, Figure 6

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Figure Caption

Figure 6

Dox-inducible CD133 expression in BAK-P cells as verified by qPCR (a) and immunoblot analysis (b). (c) Dox-inducible CD133 expression significantly increases cell invasion in transwell assays. (d) Immunoblot analysis with antibodies to CD133, MMP9, MMP2, or β-Actin for loading controls, show increased levels of CD133 as well as MP2 and MMP9 secreted by Dox-induced cells. (e) Zebrafish assays reveal enhanced metastasis in Dox-induced cells. (f,g left panels) Invasion assay results with other melanoma cell lines POT (f) and SK-MEL2 (g) CD133 CRISPR-cas9 SC versus T3 lines are consistent with results with BAK-P cells, showing that knockdown of CD133 (f,g right panels) results in decreased cell invasion. *, **, *** represent p < 0.05 and p < 0.01, and p < 0.001, respectively.

Acknowledgments

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