ZFIN ID: ZDB-FIG-190723-1305
Marra et al., 2019 - Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development. Scientific Reports   9:6454 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Figure 5

irx2a genetic mutants do not have a renal or eye phenotype. (A) Schematic of the irx2a transcript and location of the irx2asa10716 mutation, where grey is non-coding and black represents coding sequence. The forward arrow demarcates the ATG start site. (B) WISH analysis at the 28 ss shows odf3b and slc12a3 expression in irx2asa10716+/+, irx2asa10716+/−, and irx2asa10716−/− embryos. The black lines highlight the DL marker slc12a3. An enlarged dorsal view of the odf3b+ MCCs for each embryo is shown in the inset. Scale bars are 50 μm. (C) Gel image of the restriction digest product for each genotype. Wild-type bands are marked with a blue star, and mutant bands with a red star. (D) Quantification of odf3b+ cells at the 28 ss. Each dot represents one pronephros. (E) Quantification of the slc12a3 length (μm) at the 28 ss. Each dot represents one nephron. (F) Quantification of eye area (μm2). Each dot represents one eye. For all graphs, data is presented +/− SEM and statistical significance was determined by ANOVA. WISH – whole mount in situ hybridization, ss – somite stage, DL – distal late, MCC – multiciliated cell.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
odf3b TU standard conditions Prim-5 pronephric tubule multi-ciliated epithelial cell ISH
irx2asa10776/+ standard conditions Prim-5 pronephric tubule multi-ciliated epithelial cell ISH
irx2asa10776/sa10776 standard conditions Prim-5 pronephric tubule multi-ciliated epithelial cell ISH
slc12a3 TU standard conditions Prim-5 pronephric distal late tubule ISH
irx2asa10776/+ standard conditions Prim-5 pronephric distal late tubule ISH
irx2asa10776/sa10776 standard conditions Prim-5 pronephric distal late tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
irx2asa10776/+ standard conditions Prim-5 embryonic camera-type eye development normal process quality, normal
Prim-5 eye normal area, normal
Prim-5 pronephric distal late tubule normal length, normal
Prim-5 pronephric tubule has normal numbers of parts of type pronephric tubule multi-ciliated epithelial cell, normal
Prim-5 pronephric tubule multi-ciliated epithelial cell differentiation normal occurrence, normal
Prim-5 pronephros development normal process quality, normal
irx2asa10776/sa10776 standard conditions Prim-5 embryonic camera-type eye development normal process quality, normal
Prim-5 eye normal area, normal
Prim-5 pronephric distal late tubule normal length, normal
Prim-5 pronephric tubule has normal numbers of parts of type pronephric tubule multi-ciliated epithelial cell, normal
Prim-5 pronephric tubule multi-ciliated epithelial cell differentiation normal occurrence, normal
Prim-5 pronephros development normal process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.