ZFIN ID: ZDB-FIG-190723-1302
Marra et al., 2019 - Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development. Scientific Reports   9:6454 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Figure 3

irx2a acts upstream of etv5a. WISH at the 28 ss revealed decreased etv5a expression in the pronephros of irx2a morphants. Black bars denote the etv5a expression domain. Scale bar is 50 μm. (B) Quantification of etv5a length in μm at the 28 ss. Each dot represents one nephron and data is presented +/− SEM. Significance was evaluated with an unpaired student’s T-test. (C) The irx2a domain is unchanged in etv5a morphants at the 28 ss. Solid black bars denote strong irx2a expression and the dashed black lines denote faint expression in the pronephros. (D) Quantification of irx2a length (μm) at the 28 ss. Each dot represents one nephron and data is presented +/− SEM. An unpaired student’s T-test was used to determine significance. WISH – whole mount in situ hybridization, ss – somite stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv5a TU control Prim-5 pronephric tubule ISH
TU + MO2-irx2a control Prim-5 pronephric tubule ISH
irx2a TU control Prim-5 pronephric tubule ISH
TU + MO1-etv5a standard conditions Prim-5 pronephric tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-irx2a control Prim-5 pronephric tubule etv5a expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.