ZFIN ID: ZDB-FIG-190723-1303
Marra et al., 2019 - Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development. Scientific Reports   9:6454 Full text @ Sci. Rep.
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
Knockdown Reagent:
Observed In:
Stage: Prim-5

Figure 4

irx2a is required upstream of etv5a for MCC genesis. (A) Dorsal view of odf3b in the pronephros by WISH at the 28 ss shows a decrease in maturing MCCs in irx2a deficient embryos, which was rescued with irx2a cRNA, etv5a cRNA, and irx2asa10776 cRNA. Scale bar is 50 μm. (B) Quantification of odf3b+ MCCs at the 28 ss. Each dot represents one pronephros. Data is presented +/− SEM and statistical significance was determined with ANOVA. WISH – whole mount in situ hybridization, ss – somite stage, MCC – multiciliated cell.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
odf3b TU control Prim-5 pronephric tubule multi-ciliated epithelial cell ISH
TU + MO2-irx2a standard conditions Prim-5 pronephric tubule multi-ciliated epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-irx2a standard conditions Prim-5 pronephric tubule has fewer parts of type pronephric tubule multi-ciliated epithelial cell, abnormal
Prim-5 pronephric tubule multi-ciliated epithelial cell differentiation decreased occurrence, abnormal
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