ZFIN ID: ZDB-FIG-190717-2
Zhang et al., 2019 - Establishment of a zebrafish hematological disease model induced by 1,4-benzoquinone. Disease models & mechanisms   12(3) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2

Expression of lineage-specific markers in zebrafish was detected using WISH and SB staining. (A,B) Loss of βe1-globin (n=20) and rag1(control group n=37, BQ group n=49) expression in 5 dpf embryos exposed to BQ. The red boxed regions show magnified views of the caudal hematopoietic tissue (CHT). Relative rag1+ thymocyte-signal areas were analyzed using ImageJ software. The circled regions show the thymus. (C) Increase in l-plastin expression upon BQ exposure at 3 dpf (control group n=25, BQ group n=30). (D) Expression of the macrophage marker mfap4 was reduced in the BQ exposure group (control group n=52, BQ group n=76). (E,G) The neutrophil markers mpx (E) (control group n=20, BQ group n=33) and lyz (G) (control group n=39, BQ group n=35) both showed increased expression in embryos at 3 dpf following BQ exposure. (F) Increased SB+ cells were observed in the BQ exposure group compared with the control group (n=21 per group). (H–N) Quantification of WISH and SB staining for A–E (Student's t-test, mean±s.d., **P<0.01, ***P<0.001). (O) Dot plot of flow cytometry analysis of lyz:GFP+ cells from control (left) and BQ-treated fish (right). Three independent experiments (in each group, 100 embryos are pooled together) were conducted. (P) Percentage of lyz+ cells in each group [0.30% in the control group and 0.40% in the BQ group; Chi-squared test (95% c.i.), ***P<0.001].

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hbbe1.1 WT control Day 5 erythroid lineage cell ISH
WT chemical treatment by environment: 1,4-benzoquinone Day 5 caudal hematopoietic tissue ISH
Day 5 erythroid lineage cell ISH
lcp1 WT control Protruding-mouth myeloid cell ISH
WT chemical treatment by environment: 1,4-benzoquinone Protruding-mouth caudal hematopoietic tissue ISH
Protruding-mouth myeloid cell ISH
lyz WT control Protruding-mouth granulocyte ISH
WT chemical treatment by environment: 1,4-benzoquinone Protruding-mouth caudal hematopoietic tissue ISH
Protruding-mouth granulocyte ISH
mfap4 WT control Protruding-mouth macrophage ISH
WT chemical treatment by environment: 1,4-benzoquinone Protruding-mouth caudal hematopoietic tissue ISH
Protruding-mouth macrophage ISH
mpx WT control Protruding-mouth granulocyte ISH
WT chemical treatment by environment: 1,4-benzoquinone Protruding-mouth caudal hematopoietic tissue ISH
Protruding-mouth granulocyte ISH
rag1 WT control Day 5 lymphoid progenitor cell ISH
WT chemical treatment by environment: 1,4-benzoquinone Day 5 lymphoid progenitor cell ISH
Day 5 thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: 1,4-benzoquinone Protruding-mouth caudal hematopoietic tissue mfap4 expression decreased amount, abnormal
Protruding-mouth caudal hematopoietic tissue mpx expression increased amount, abnormal
Protruding-mouth caudal hematopoietic tissue lyz expression increased amount, abnormal
Protruding-mouth caudal hematopoietic tissue lcp1 expression increased amount, abnormal
Protruding-mouth lymphoid progenitor cell decreased amount, abnormal
Protruding-mouth macrophage decreased amount, abnormal
Protruding-mouth myeloid cell decreased amount, abnormal
Protruding-mouth neutrophil increased amount, abnormal
Day 5 caudal hematopoietic tissue hbbe1.1 expression decreased amount, abnormal
Day 5 erythroid lineage cell decreased amount, abnormal
Day 5 lymphoid progenitor cell decreased amount, abnormal
Day 5 neutrophil increased amount, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
nz117Tg chemical treatment by environment: 1,4-benzoquinone Protruding-mouth neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.