ZFIN ID: ZDB-FIG-190715-21
Toms et al., 2019 - Phagosomal and mitochondrial alterations in RPE may contribute to KCNJ13 retinopathy. Scientific Reports   9:3793 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 8

Retinal stress in obetd15 zebrafish. Immunostaining of the retina at 3, 6 and 12 mpf using anti-ZRF1 to detect expression of glial fibrillary acidic protein (red), counterstained with DAPI nucleic acid stain (blue) (a). Quantitative RT-PCR was used to determine relative mRNA expression levels of sod1 (b), sod2 (c) and bcl2a (d) genes at 6 and 12 mpf (n = 3 for each age, mean ± SEM). Western blot analysis of heat shock protein 60 (hsp60) expression (e). The blot was re-probed with anti-β-actin as a loading control for samples. Bands of interest are cropped from full-length blot presented in Supplementary Fig. S4. The bar chart (f) shows mean ± SEM result from three independent experiments. *p < 0.05. Scale bar = 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bcl2a AB standard conditions Adult retina RTPCR
kcnj13td15/td15 standard conditions Adult retina RTPCR
sod1 AB standard conditions Adult retina RTPCR
kcnj13td15/td15 standard conditions Adult retina RTPCR
sod2 AB standard conditions Adult retina RTPCR
kcnj13td15/td15 standard conditions Adult retina RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zrf-1 IHC AB standard conditions Adult Muller cell cell projection
IHC Adult Muller cell glial limiting end-foot
IHC kcnj13td15/td15 standard conditions Adult Muller cell cell projection
IHC Adult Muller cell glial limiting end-foot
IHC Adult retinal outer nuclear layer
Phenotype Details
Fish Conditions Stage Phenotype
kcnj13td15/td15 standard conditions Adult Muller cell cell projection zrf-1 labeling increased distribution, abnormal
Adult retina bcl2a expression increased amount, abnormal
Adult retina sod1 expression increased amount, abnormal
Adult retina sod2 expression increased amount, abnormal
Adult retinal outer nuclear layer zrf-1 labeling mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.