FIGURE

Fig. 5

ID
ZDB-FIG-190708-52
Publication
Kujawski et al., 2019 - penner/lgl2 is required for the integrity of the photoreceptor layer in the zebrafish retina
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Fig. 5

Knockdown of Lgl2 in pen/lgl2 clutches leads to disorganization of retinal lamination. Immunostaining of transverse retinal sections at 5 dpf of MO-injected and genotyped pen/lgl2 clutches. (A–C″′) CNTR-MO-injected fish and (D-F′′′′) ATG-MO1-injected fish. Zpr1 (A–F) stains double cone cell bodies and WGA (A′–F′) the OPL and OSs. In ATG-MO1-injected hetero- (E–E′′′′) or homozygous (F–F′′′′) fish, layering in the distal retina is disturbed. Arrows in E′, E″, F′ and F″ mark disorganized clusters separated by normally aligned cells (asterisk). Boxed areas in D′′′–F′′′ show enlarged views of PRCs in MO-injected pen/lgl2 WT (D′′′′), heterozygote (E′′′′) and mutant (F′′′′) fish. Occasionally Zpr1 positive cells were seen outside of the PRC layer (E,E′′′′, arrowheads). Arrowheads in F′′′′ mark apically displaced PRCs that are localized on top of another PRC. PRC, photoreceptor layer; OPL, outer plexiform layer; INL, inner nuclear layer. Scale bar: 100 µm.

Expression Data
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
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